Arg-Gly-Asp-D-Phe-Lys peptide-modified PEGylated dendrimer-entrapped gold nanoparticles for targeted computed tomography imaging of breast carcinoma.

Abstract

AIM:

To investigate cyclo (Arg-Gly-Asp-D-Phe-Lys) peptide (RGD)-modified PEGylated dendrimer-entrapped gold nanoparticles (PEGylated Au DENPs-RGD) for targeted computed tomography (CT) imaging of breast carcinomas.

MATERIALS & METHODS:

PEGylated Au DENPs-RGD were synthesized and characterized. Then, the PEGylated Au DENPs-RGD for targeted CT imaging were investigated using the MDA-MB-435 cell line, an integrin-rich breast carcinoma cells, and mice with MDA-MB-435 xenograft tumors. Finally, silver enhancement staining and integrin αvβ3 immunohistochemistry of the tumors were performed.

RESULTS:

The synthesized PEGylated Au DENPs-RGD were spherical, water dispersible and biocompatible nanoprobes with a gold nanoparticle core size of 2.8 nm. Due to the presence of the Au nanoparticles, the PEGylated Au DENPs-RGD displayed a higher x-ray attenuation intensity than Omnipaque at the same Au or I concentrations. The conjugated RGD ligand can specifically identify and target overexpressed integrin receptors on MDA-MB-435 cells. After intravenous injection, these nanoprobes accumulated in the targeted area of mice with MDA-MB-435 xenograft tumors, which enabled the tumor to be detected by CT imaging. The histological results confirmed the imaging results.

CONCLUSION:

The PEGylated Au DENPs-RGD can be used as targeted nanoprobes with good biocompatibility for targeted CT imaging and diagnosis of integrin-positive tumors.

• Authors: Kangan Li, Zhuoli Zhang, Linfeng Zheng, Hong Liu, Wei Wei, Zhiyu Li, Zhiyan He, Andrew C Larson, Guixiang Zhang
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 10 Issue 14 Pg. 2185-97 (Jul 2015) ISSN: 1748-6963 [Electronic] England
• PMID: 26214356 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dendrimers
• Dipeptides
• Oligopeptides
• lysylphenylalanine
• Gold
• arginyl-glycyl-aspartic acid

Topics

• Animals
• Breast Neoplasms (diagnostic imaging)
• Cell Line, Tumor
• Dendrimers (chemistry)
• Dipeptides (chemistry)
• Female
• Gold (chemistry)
• Humans
• Immunohistochemistry
• Metal Nanoparticles (chemistry)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Oligopeptides (chemistry)
• Tomography, X-Ray Computed (methods)