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Murine K2P5.1 Deficiency Has No Impact on Autoimmune Neuroinflammation due to Compensatory K2P3.1- and KV1.3-Dependent Mechanisms.

Abstract
Lymphocytes express potassium channels that regulate physiological cell functions, such as activation, proliferation and migration. Expression levels of K2P5.1 (TASK2; KCNK5) channels belonging to the family of two-pore domain potassium channels have previously been correlated to the activity of autoreactive T lymphocytes in patients with multiple sclerosis and rheumatoid arthritis. In humans, K2P5.1 channels are upregulated upon T cell stimulation and influence T cell effector functions. However, a further clinical translation of targeting K2P5.1 is currently hampered by a lack of highly selective inhibitors, making it necessary to evaluate the impact of KCNK5 in established preclinical animal disease models. We here demonstrate that K2P5.1 knockout (K2P5.1-/-) mice display no significant alterations concerning T cell cytokine production, proliferation rates, surface marker molecules or signaling pathways. In an experimental model of autoimmune neuroinflammation, K2P5.1-/- mice show a comparable disease course to wild-type animals and no major changes in the peripheral immune system or CNS compartment. A compensatory upregulation of the potassium channels K2P3.1 and KV1.3 seems to counterbalance the deletion of K2P5.1. As an alternative model mimicking autoimmune neuroinflammation, experimental autoimmune encephalomyelitis in the common marmoset has been proposed, especially for testing the efficacy of new potential drugs. Initial experiments show that K2P5.1 is functionally expressed on marmoset T lymphocytes, opening up the possibility for assessing future K2P5.1-targeting drugs.
AuthorsStefan Bittner, Nicole Bobak, Majella-Sophie Hofmann, Michael K Schuhmann, Tobias Ruck, Kerstin Göbel, Wolfgang Brück, Heinz Wiendl, Sven G Meuth
JournalInternational journal of molecular sciences (Int J Mol Sci) Vol. 16 Issue 8 Pg. 16880-96 (Jul 24 2015) ISSN: 1422-0067 [Electronic] Switzerland
PMID26213925 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Kv1.3 Potassium Channel
  • Potassium Channels, Tandem Pore Domain
  • Receptors, Antigen, T-Cell
Topics
  • Animals
  • Callithrix
  • Disease Models, Animal
  • Disease Progression
  • Encephalomyelitis, Autoimmune, Experimental (metabolism, pathology)
  • Gene Deletion
  • Immune System (pathology)
  • Kv1.3 Potassium Channel (metabolism)
  • Mice
  • Mice, Knockout
  • Phenotype
  • Potassium Channels, Tandem Pore Domain (metabolism)
  • Receptors, Antigen, T-Cell (metabolism)
  • Signal Transduction
  • T-Lymphocytes (immunology)
  • Up-Regulation

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