Abstract | RATIONALE:
Glycine is the smallest amino acid used in protein synthesis, but it is also a very important precursor for the biosynthesis of other nitrogen-containing metabolites, such as purine nucleosides and nucleotides for synthesis of RNA, DNA etc. Abnormalities in glycine metabolism therefore cause diseases such as cancer. A quick and unambiguous method to trace the metabolites arising from glycine is required for targeting defect points within metabolic networks. METHODS: This paper describes a method for using (15)N-glycine to culture A549 cancer cells for use with high-resolution mass spectrometry (HRMS) and tandem mass spectrometry (HRMS(2)) that can detect the (M+1)/M pair peaks appearing in the cell metabolites. The 1 Da difference in the pair peaks can be used to point out and identify the nitrogen metabolites of glycine. RESULTS: CONCLUSIONS: The method developed here could be applied to investigations of metabolism of other amino acids, and for drug discovery studies targeting the enzymes related to amino acid metabolism.
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Authors | Lin-na Liu, Ting-ting Fu, Xun-fu Xu, Chuan Fu, Mei-juan Fang, Yan Liu, Peng-xiang Xu, Yu-fen Zhao |
Journal | Rapid communications in mass spectrometry : RCM
(Rapid Commun Mass Spectrom)
Vol. 29
Issue 7
Pg. 645-53
(Apr 15 2015)
ISSN: 1097-0231 [Electronic] England |
PMID | 26212282
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 John Wiley & Sons, Ltd. |
Chemical References |
- Ions
- Nitrogen Isotopes
- Glycine
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Topics |
- Cell Line
- Glycine
(analysis, chemistry, metabolism)
- Humans
- Ions
(analysis, chemistry)
- Metabolomics
- Nitrogen Isotopes
(analysis, chemistry, metabolism)
- Tandem Mass Spectrometry
(methods)
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