Cigarette
smoke has been reported to be the major cause of
chronic obstructive pulmonary disease (
COPD). It causes persistent
inflammation by regulating the redox-sensitive pathways.
Forsythiaside, an active constituent isolated from the Chinese medicinal herb Forsythia suspensa, has been reported to have anti-inflammatory and
anti-oxidant effects. Thus, in this study, we investigated the protective effects of
forsythiaside against cigarette
smoke-induced
lung inflammation in mice.
COPD mice model was established by cigarette
smoke.
Forsythiaside was given 2h before cigarette
smoke exposure for five consecutive days. Bronchoalveolar lavage fluid and lung tissues were collected to assess pathological changes, lipid peroxidation, inflammatory
cytokine production, Nrf-2, and NF-κB expression. Our results showed that
forsythiaside attenuated the infiltration of inflammatory cells, NO and inflammatory
cytokines TNF-α,
IL-6 and IL-1β production, and reversed the CS-induced decrease of
glutathione/
glutathione disulfide (GSH/
GSSG) ratio. Western blot analysis showed that
forsythiaside inhibited cigarette
smoke-induced NF-κB activation. In addition,
forsythiaside dose-dependently up-regulated the expression of Nrf2 and HO-1. In conclusion,
forsythiaside protected against cigarette
smoke-induced
lung injury through activating Nrf2 and inhibiting NF-κB signaling pathway.