Abstract | BACKGROUND: METHODS: RESULTS: By genome-wide RNA-seq of tongue samples (3 mice per group), we identified changes in transcripts that mediate alcohol metabolism and oxidative stress (Aldh2, Aldh1a3, Adh1, Adh7, and Cyp2a5) in mice treated with 4-NQO followed by ethanol (4-NQO/EtOH) as compared to the vehicle control/untreated (V.C./Untr.) samples. We measured major, global increases in specific histone acetylation and methylation epigenetic marks (H3K27ac, H3K9/14ac, H3K27me3, and H3K9me3) in the oral cavities of V.C./EtOH, 4-NQO/Untr., and 4-NQO/EtOH treatment groups compared to the V.C./Untr. group. We detected changes in histone epigenetic marks near regulatory regions of genes involved in ethanol metabolism by chromatin immunoprecipitation. For instance, the Aldh2 promoter showed increased H3K27me3 marks, and Aldh2 mRNA levels were reduced by 10-fold in 4NQO/EtOH versus V.C./Untr. tongue samples. 4-NQO/EtOH treatment also caused increases in markers of oxidative stress, including 4-HNE, MCT4/SLC16a3, and TOM20, as measured by immunohistochemistry. CONCLUSIONS: We delineate a mechanism by which 4-NQO and ethanol can regulate gene expression during the development of HNSCC and suggest that histone epigenetic marks and oxidative stress markers could be the novel biomarkers and targets for the prevention of HNSCC.
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Authors | Alison M Urvalek, Kwame Osei-Sarfo, Xiao-Han Tang, Tuo Zhang, Theresa Scognamiglio, Lorraine J Gudas |
Journal | Alcoholism, clinical and experimental research
(Alcohol Clin Exp Res)
Vol. 39
Issue 8
Pg. 1360-72
(Aug 2015)
ISSN: 1530-0277 [Electronic] England |
PMID | 26207766
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2015 by the Research Society on Alcoholism. |
Chemical References |
- Biomarkers, Tumor
- Carcinogens
- Ethanol
- 4-Nitroquinoline-1-oxide
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Topics |
- 4-Nitroquinoline-1-oxide
(toxicity)
- Animals
- Biomarkers, Tumor
(metabolism)
- Carcinogenesis
- Carcinogens
(toxicity)
- Epigenesis, Genetic
(drug effects, physiology)
- Ethanol
(toxicity)
- Female
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mouth Neoplasms
(metabolism, pathology)
- Oxidative Stress
(drug effects, physiology)
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