Chronic unloading induces decrements in muscle size and strength. This adaptation is governed by a number of molecular factors including
myostatin, a potent negative regulator of muscle mass.
Myostatin must first be secreted into the circulation and then bind to the membrane-bound
activin receptor IIB (actRIIB) to exert its atrophic action. Therefore, we hypothesized that myofiber type-specific
atrophy observed after hindlimb suspension (HLS) would be related to myofiber type-specific expression of
myostatin and/or actRIIB. Wistar rats underwent HLS for 10 days, after which the tibialis anterior was harvested for frozen cross sectioning. Simultaneous multichannel immunofluorescent staining combined with differential interference contrast imaging was employed to analyze myofiber type-specific expression of
myostatin and actRIIB and myofiber type cross-sectional area (CSA) across fiber types, myonuclei, and satellite cells. Hindlimb suspension (HLS) induced significant myofiber type-specific
atrophy in
myosin heavy chain (MHC) IIx (P < 0.05) and MHC IIb myofibers (P < 0.05).
Myostatin staining associated with myonuclei was less in HLS rats compared with controls, while satellite cell staining for
myostatin remained unchanged. In contrast, the total number myonuclei and satellite cells per myofiber was reduced in HLS compared with ambulatory control rats (P < 0.01). Sarcoplasmic actRIIB staining differed between myofiber types (I < IIa < IIx < IIb) independent of loading conditions. Myofiber types exhibiting the greatest cytoplasmic staining of actRIIB corresponded to those exhibiting the greatest degree of
atrophy following HLS. Our data suggest that differential expression of actRIIB may be responsible for
myostatin-induced myofiber type-selective
atrophy observed during chronic unloading.