Targeted Next-Generation Sequencing Reveals Hot Spots and Doubly Heterozygous Mutations in Chinese Patients with Familial Cardiomyopathy.

Abstract

As a common cardiac disease mainly caused by gene mutations in sarcomeric cytoskeletal, calcium-handling, nuclear envelope, desmosomal, and transcription factor genes, inherited cardiomyopathy is becoming one of the major etiological factors of sudden cardiac death (SCD) and heart failure (HF). This disease is characterized by remarkable genetic heterogeneity, which makes it difficult to screen for pathogenic mutations using Sanger sequencing. In the present study, three probands, one with familial hypertrophic cardiomyopathy (FHCM) and two with familial dilated cardiomyopathy (FDCM), were recruited together with their respective family members. Using next-generation sequencing technology (NGS), 24 genes frequently known to be related to inherited cardiomyopathy were screened. Two hot spots (TNNI3-p.Arg145Gly, and LMNA-p.Arg190Trp) and double (LMNA-p.Arg190Trp plus MYH7-p.Arg1045His) heterozygous mutations were found to be highly correlated with familial cardiomyopathy. FDCM patients with doubly heterozygous mutations show a notably severe phenotype as we could confirm in our study; this indicates that the double mutations had a dose effect. In addition, it is proposed that genetic testing using NGS technology can be used as a cost-effective screening tool and help guide the treatment of patients with familial cardiomyopathy particularly regarding the risk of family members who are clinically asymptomatic.

• Authors: Yue Zhao, Yue Feng, Yun-Mei Zhang, Xiao-Xue Ding, Yu-Zhu Song, A-Mei Zhang, Li Liu, Hong Zhang, Jia-Huan Ding, Xue-Shan Xia
• Journal: BioMed research international (Biomed Res Int) Vol. 2015 Pg. 561819 ( 2015) ISSN: 2314-6141 [Electronic] United States
• PMID: 26199943 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• LMNA protein, human
• Lamin Type A
• MYH7 protein, human
• Troponin I
• Cardiac Myosins
• Myosin Heavy Chains

Topics

• Adult
• Asian People (genetics)
• Base Sequence
• Cardiac Myosins (genetics)
• Cardiomyopathy, Dilated (diagnostic imaging, genetics)
• China
• DNA Mutational Analysis
• Female
• Heterozygote
• High-Throughput Nucleotide Sequencing (methods)
• Humans
• Lamin Type A (genetics)
• Male
• Molecular Sequence Data
• Mutation (genetics)
• Myosin Heavy Chains (genetics)
• Phenotype
• Troponin I (genetics)
• Ultrasonography
• Young Adult