Expression of apoptosis signal-regulating kinase 1 is associated with tight junction protein claudin-6 in cervical carcinoma.

Abstract

In our previous work, apoptosis signal-regulating kinase 1 (ASK1), a member of the mitogen-activated protein kinase (MAPK) kinase family has been proved to be associated with the pro-apoptosis effect of tight junction protein claudin-6 in breast cancer. However, its expression in cervical carcinoma has not been reported. The ASK1 and claudin-6 expression in cervical carcinoma tissues and adjacent non-neoplastic tissues was examined by immunohistochemistry. The mRNA and protein expression of ASK1 and claudin-6 in cervical cancer carcinoma cells was detected by reverse transcription (RT)-PCR and western blot. The expression level of ASK1 was down-regulated in cervical carcinoma tissues compared with the adjacent non-neoplastic tissues and positively correlated with the level of claudin-6 in cervical carcinoma cells and tissues. Our present study reveals that ASK1 protein expression altered between human cervical carcinoma and adjacent non-neoplastic tissues. The expression of ASK1 is correlated with the level of claudin-6 in cervical carcinoma cells and tissues.

• Authors: Xiaowei Zhang, Yang Ruan, Yanru Li, Dongjing Lin, Zhijing Liu, Chengshi Quan
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 5 Pg. 5535-41 ( 2015) ISSN: 1936-2625 [Electronic] United States
• PMID: 26191261 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Claudins
• RNA, Messenger
• MAP Kinase Kinase Kinase 5
• MAP3K5 protein, human
• claudin 6

Topics

• Adenocarcinoma (enzymology, genetics, pathology)
• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Carcinoma, Squamous Cell (enzymology, genetics, pathology)
• Claudins (analysis, genetics)
• Down-Regulation
• Female
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Neoplastic
• HeLa Cells
• Humans
• Immunohistochemistry
• MAP Kinase Kinase Kinase 5 (genetics, metabolism)
• Middle Aged
• RNA, Messenger (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Uterine Cervical Neoplasms (enzymology, genetics, pathology)