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Melatonin Attenuates Oxidative Damage Induced by Acrylamide In Vitro and In Vivo.

Abstract
Acrylamide (ACR) has been classified as a neurotoxic agent in animals and humans. Melatonin (MT) has been shown to be potentially effective in preventing oxidative stress related neurodegenerative disorders. In this study, whether MT exerted a protective effect against ACR-induced oxidative damage was investigated. Results in cells showed that reactive oxygen species (ROS) and malondialdehyde (MDA) significantly increased after ACR treatment for 24 h. MT preconditioning or cotreatment with ACR reduced ROS and MDA products, whereas the inhibitory effect of MT on oxidant generation was attenuated by blocking the MT receptor. Increased DNA fragmentation caused by ACR was significantly decreased by MT coadministration. In vivo, rats at 40 mg/kg/day ACR by gavage for 12 days showed weight loss and gait abnormality, Purkinje cell nuclear condensation, and DNA damage in rat cerebellum. MT (i.p) cotreatment with ACR not only recovered weight and gait of rats, but also decreased nuclear condensation and DNA damage in rat cerebellum. Using MDA generation, glutathione (GSH) level, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in rat cerebellum as indicators, MT alleviated ACR-induced lipid peroxidation and depressed antioxidant capacity. Our results suggest that MT effectively prevents oxidative damage induced by ACR.
AuthorsXiaoqi Pan, Lanlan Zhu, Huiping Lu, Dun Wang, Qing Lu, Hong Yan
JournalOxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2015 Pg. 703709 ( 2015) ISSN: 1942-0994 [Electronic] United States
PMID26185593 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Reactive Oxygen Species
  • Acrylamide
  • Malondialdehyde
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • Melatonin
Topics
  • Acrylamide (toxicity)
  • Animals
  • Brain (drug effects, metabolism, pathology)
  • Cell Survival (drug effects)
  • DNA Damage (drug effects)
  • Glutathione (metabolism)
  • Glutathione Peroxidase (metabolism)
  • Lipid Peroxidation (drug effects)
  • Male
  • Malondialdehyde (metabolism)
  • Melatonin (pharmacology)
  • Oxidative Stress (drug effects)
  • PC12 Cells
  • Rats
  • Rats, Sprague-Dawley
  • Reactive Oxygen Species (metabolism)
  • Superoxide Dismutase (metabolism)

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