Abstract | BACKGROUND AND OBJECTIVE: METHODS: Appropriate concentration and time were selected with a CCK8 kit. The drug that was used in H446 cells was divided into four different concentrations: 0 μmol/L, 3.4 μmol/L, 6.8 μmol/L and 13.6 μmol/L. The changes in forms and nucleus of H446 cells that were stained with DAPI were observed under an inverted optical microscope. The effects on H446 cell apoptosis were detected by FCM. The changes in apoptosis-related proteins BCL2, BAX, and CASP3 were investigated using Western blot for 24 h. RESULTS:
Solasonine reduced the survival ratio of H446 cells and inhibited the proliferation with a dose-related effect. The survival ratio of H446 cells could be reduced to 16.77% (P<0.001), and the highest apoptosis ratio was 44.62% (P<0.001). Apoptosis was observed in H446 cells. Moreover, Western blot showed that the apoptosis-related proteins BAX and CASP3 were upregulated (P<0.05). CONCLUSIONS:
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Authors | Wensi Huang, Ying Wang, Haitao Zhu, Yingying Wu, Xiaodong Xie, Dongqing Wang |
Journal | Zhongguo fei ai za zhi = Chinese journal of lung cancer
(Zhongguo Fei Ai Za Zhi)
Vol. 18
Issue 7
Pg. 416-21
(Jul 2015)
ISSN: 1999-6187 [Electronic] China |
PMID | 26182866
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Proto-Oncogene Proteins c-bcl-2
- Solanaceous Alkaloids
- alpha-solamargine
- Caspase 3
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Caspase 3
(genetics, metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Down-Regulation
(drug effects)
- Humans
- Lung Neoplasms
(drug therapy, genetics, metabolism, physiopathology)
- Proto-Oncogene Proteins c-bcl-2
(genetics, metabolism)
- Solanaceous Alkaloids
(pharmacology)
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