Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia-Reperfusion Injury in Mice.

Abstract

Oxysophoridine (OSR) is a bioactive alkaloid extracted from the Sophora alopecuroides Linn. Our aim is to explore the potential anti-inflammation mechanism of OSR in cerebral ischemic injury. Mice were intraperitoneally pretreated with OSR (62.5, 125, and 250 mg/kg) or nimodipine (Nim) (6 mg/kg) for 7 days followed by cerebral ischemia. The inflammatory-related cytokines in cerebral ischemic hemisphere tissue were determined by immunohistochemistry staining, Western blot and enzyme-like immunosorbent assay (ELISA). OSR-treated groups observably suppressed the nuclear factor kappa B (NF-κB), intercellular adhesion molecule-1 (ICAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). OSR-treated group (250 mg/kg) markedly reduced the inflammatory-related protein prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8). Meanwhile, it dramatically increased the interleukin-10 (IL-10). Our study revealed that OSR protected neurons from ischemia-induced injury in mice by downregulating the proinflammatory cytokines and blocking the NF-κB pathway.

• Authors: Yong-Sheng Wang, Yu-Xiang Li, Peng Zhao, Hong-Bo Wang, Ru Zhou, Yin-Ju Hao, Jie Wang, Shu-Jing Wang, Juan Du, Lin Ma, Tao Sun, Jian-Qiang Yu
• Journal: Inflammation (Inflammation) Vol. 38 Issue 6 Pg. 2259-68 (Dec 2015) ISSN: 1573-2576 [Electronic] United States
• PMID: 26178478 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Anti-Inflammatory Agents
• Cytokines
• Icam1 protein, mouse
• Inflammation Mediators
• Neuroprotective Agents
• Rela protein, mouse
• Transcription Factor RelA
• oxysophoridine
• Intercellular Adhesion Molecule-1
• Nitric Oxide
• Nitric Oxide Synthase Type II
• Nos2 protein, mouse
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• Dinoprostone

Topics

• Alkaloids (pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Brain (drug effects, metabolism, pathology)
• Brain Ischemia (drug therapy, metabolism, pathology)
• Cyclooxygenase 2 (metabolism)
• Cytokines (metabolism)
• Dinoprostone (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Inflammation Mediators (metabolism)
• Intercellular Adhesion Molecule-1 (metabolism)
• Male
• Mice, Inbred ICR
• Neuroprotective Agents (pharmacology)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Signal Transduction (drug effects)
• Time Factors
• Transcription Factor RelA (metabolism)