Genetic association of complement component 2 polymorphism with systemic lupus erythematosus.

Abstract

Complement component 2 (C2), an early member of the classical pathway, mainly participates in apoptotic cell clearance. We hypothesize that C2 polymorphism may confer genetic susceptibility to complement dysfunction in systemic lupus erythematosus (SLE). The major aim of our study was to investigate the clinical and serological associations of C2 variants in Chinese patients with SLE. The single-nucleotide polymorphism (rs2844455, G/A SNP) located in the intron region of C2 gene was genotyped by direct sequencing in 95 SLE patients and 95 matched normal control subjects. The gene expression profiles were generated by quantitative real-time polymerase chain reaction (PCR) and reverse transcription PCR. Our results showed that the AA genotype was observed more frequently in SLE patients than in normal control subjects (22.1% vs 9.5%, P < 0.05). The A allele was strongly associated with the occurrence of hair loss, photosensitivity and anti-cardiolipin antibodies; whereas, the G allele was associated with lower frequencies of these clinical presentations. Relative expression levels were significantly lower in patients with the AA genotype [median: 18.86, interquartile range (IQR) 11.36-22.43, P = 0.002] than in those with the GG genotype (35.76, IQR: 19.33-49.71). As expected, we confirmed the A allele as a risk factor for SLE development in a Chinese population, in contrast, the G allele might be a protective factor against the pathogenic autoantibody formation and cutaneous manifestations in SLE patients.

• Authors: H-H Chen, L-J Tsai, K-R Lee, Y-M Chen, W-T Hung, D-Y Chen
• Journal: Tissue antigens (Tissue Antigens) Vol. 86 Issue 2 Pg. 122-33 (Aug 2015) ISSN: 1399-0039 [Electronic] England
• PMID: 26176736 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Antibodies, Anticardiolipin
• Complement C2

Topics

• Adult
• Alleles
• Alopecia (ethnology, genetics, immunology, pathology)
• Antibodies, Anticardiolipin (blood)
• Asian People
• Case-Control Studies
• Complement C2 (genetics, immunology)
• Exons
• Female
• Gene Expression
• Gene Expression Profiling
• Gene Frequency
• Genetic Association Studies
• Genetic Predisposition to Disease
• Genotype
• Humans
• Introns
• Lupus Erythematosus, Systemic (ethnology, genetics, immunology, pathology)
• Male
• Middle Aged
• Photophobia (ethnology, genetics, immunology, pathology)
• Polymorphism, Single Nucleotide