Abstract | INTRODUCTION: Expression of Klotho is decreased and endoplasmic reticulum (ER) stress is activated in patients with chronic kidney disease. This study aimed to investigate the effect of Klotho protein on ER stress-related apoptosis and renal fibrosis in rates with unilateral ureteral obstruction (UUO). MATERIALS AND METHODS: RESULTS: Kidney dysfunction, increased renal injury index, and aggravated renal fibrosis were documented in the UUO group. Expression of Klotho in the UUO rats was remarkably decreased (P < .05) and expression of all three ER stress-related proteins were significantly upregulated, accompanied by increasing numbers of apoptotic cells (P < .05). Soluble Klotho administration improved kidney function, ameliorated pathological changes, decreased expressions of ER-stress related- proteins, and reduced the number of apoptotic cells. CONCLUSION: Unilateral ureteral obstruction decreased Klotho expression and activated ER stress and related apoptosis. Klotho administration ameliorated UUO-induced ER stress, inhibited apoptotic process, and attenuated renal fibrosis.
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Authors | Qi-Feng Liu, Jian-Ming Ye, Zhi-Yong Deng, Li-Xia Yu, Qiang Sun, Sha-Sha Li |
Journal | Iranian journal of kidney diseases
(Iran J Kidney Dis)
Vol. 9
Issue 4
Pg. 291-7
(Jul 2015)
ISSN: 1735-8604 [Electronic] Iran |
PMID | 26174456
(Publication Type: Journal Article)
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Chemical References |
- Glucuronidase
- Klotho Proteins
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Topics |
- Animals
- Apoptosis
(drug effects)
- Disease Models, Animal
- Endoplasmic Reticulum Stress
(drug effects)
- Fibrosis
- Glucuronidase
(administration & dosage)
- Kidney
(pathology)
- Klotho Proteins
- Male
- Rats
- Rats, Sprague-Dawley
- Ureteral Obstruction
(complications)
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