Abstract |
The metastatic status of oral cancer is highly associated with the overall survival rate of patients. Previous studies have revealed that the endogenous tryptophan metabolite 5-methoxytryptophan (5-MTP) can downregulate cyclooxygenase-2 expression; suppress tumor proliferation, migration, and invasion; and reduce the tumor size. To improve the understanding of the molecular mechanisms involved in the regulation of 5-MTP in the tumorigenesis of oral cancer, we conducted a comparative wound healing and transwell invasion assays. Our results revealed that 5-MTP reduce oral cancer cell migration and invasion ability. In addition, the results of an in vivo assay demonstrated that the growth of primary tumors was significantly inhibited by 5-MTP in OC3 oral cancer cells and in invasive OC3-I5 oral cancer cells. Moreover, enlarged spleens were observed in OC3-I5-implanted severe combined immunodeficiency mice although 5-MTP can inhibit spleen enlargement. Through comparative proteomics, we identified 32 differentially regulated protein spots by using 2D-DIGE/MALDI-TOF MS analyses. Some of the differentially regulated proteins such as amadillo-repeat-containing X-linked protein 1, phosphoglycerate kinase 1, tropomyosin alpha-1, and tropomyosin alpha-4 may be associated with the 5-MTP-dependent inhibition of oral cancer growth and metastasis. We conclude that 5-MTP plays a crucial role in inhibiting in vitro and in vivo cancer invasion and metastasis.
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Authors | Ssu-Han Wang, Chien-Wen Chang, Hsiu-Chuan Chou |
Journal | Electrophoresis
(Electrophoresis)
Vol. 36
Issue 17
Pg. 2027-34
(Sep 2015)
ISSN: 1522-2683 [Electronic] Germany |
PMID | 26171676
(Publication Type: Journal Article)
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Copyright | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Antineoplastic Agents
- Proteome
- 5-methoxytryptophan
- Tryptophan
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Female
- Humans
- Mice
- Mice, Nude
- Mouth Neoplasms
(metabolism, pathology)
- Neoplasm Invasiveness
- Proteome
(analysis, drug effects)
- Proteomics
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Tryptophan
(analogs & derivatives, pharmacology)
- Two-Dimensional Difference Gel Electrophoresis
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