Whey Protein Hydrolysate but not Whole Whey Protein Protects Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Rats.

Abstract

Effects of intact and processed bovine milk proteins on development of chemically induced mammary tumors in female rats were compared. AIN-93G diets were made with 20% casein (CAS), casein hydrolysate (CASH), intact whey protein (IWP), or whey protein hydrolysate (WPH). Pregnant Sprague-Dawley rats were fed the diets starting at Gestational Day 4. Offspring were fed the same diet. At 50 days, female offspring (44-49/group) were gavaged with sesame oil containing 80 mg/kg of the mammary carcinogen dimethylbenzanthracene (DMBA) and euthanized 62 days posttreatment. Rats fed WPH had an adenocarcinoma incidence of 17% compared to the rats fed CAS, CASH, and IWP diets (34%, 33%, and 36% respectively) (P < 0.001). Median palpable tumor latency for rats fed WPH was greater (61 days, P < 0.001) compared to CAS (44 days), CASH (42 days) and IWP (45 days). Tumor multiplicity was also lower (1.5 vs. 3.0, P < 0.05) in rats fed WPH than in CAS and CASH fed groups. Results demonstrate that hydrolytic processing of whey protein is required for this diet to be effective in reducing DMBA-induced mammary tumors. The bioactive compounds produced during whey protein processing and mechanisms underlying the anticancer effects of WPH are yet to be identified.

• Authors: Martin J Ronis, Reza Hakkak, Soheila Korourian, Thomas M Badger
• Journal: Nutrition and cancer (Nutr Cancer) Vol. 67 Issue 6 Pg. 949-53 ( 2015) ISSN: 1532-7914 [Electronic] United States
• PMID: 26168336 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Carcinogens
• Caseins
• Protective Agents
• Protein Hydrolysates
• Whey Proteins
• 9,10-Dimethyl-1,2-benzanthracene
• casein hydrolysate

Topics

• 9,10-Dimethyl-1,2-benzanthracene (toxicity)
• Animals
• Carcinogens (toxicity)
• Caseins (pharmacology)
• Diet
• Disease Models, Animal
• Female
• Mammary Neoplasms, Animal (chemically induced, drug therapy)
• Pregnancy
• Protective Agents (pharmacology)
• Protein Hydrolysates (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Whey Proteins (pharmacology)