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[Isolation, Identification and Analysis of the Complete Genome Sequence of Black Queen Cell Virus Strain China-JL1].

Abstract
Honeybee pupae were collected from Jilin apiaries and RNA was extracted for use as a tefnplate for amplification. Based on the complete genome sequences of black queen cell virus (BQCV) published on GenBank, we designed 10 pairs of primers to amplify genes by reverse transcription-polymerase chain reaction (RT-PCR). Using this approach, we have obtained the first complete genome sequence of a BQCV isolate in China. The genome of the isolated strain, named BQCV-JL1, is composed of 8358 nucleotides and shares between 86% and 93% homology with the complete genome sequences of the other six BQCV strains published on GenBank. ORF 1 of BQCV-JL1 is positioned between nucleot ides (nt) 546 and 4676 (4131 nt), while ORF 2 is located between nt 5750 and 8203. Between the two ORFs of BQCV-JL1 there is a short ORF, called ORF 3, between nt 4891 and 5433 (543 nt). The first functional gene ex- pression domain of the BQCV-JL1 strain is positioned between nt 546 and 5 429, encompassing both ORF 1 and ORF 3. There is an internal ribosome entry site (IRES) located before ORF 2, the last three bases of which are CCU (nt 5642-5644). These bases act as an initiation.codon facilitating the translation of ORF 2. The second functional gene expression domain of the BQCV-JL1 strain is located between nt positions 5642 and 8203. The BQCV-JL1 strain was found to share high sequence identity (93%) with the Hungary 10 genotype at the whole-genome level and analysis of the nucleotide and amino acid sequences revealed that the BQCV-JL1 strain also shows close genetic relationships with the South Korea strain, suggesting that both the BQCV-JL1 and South Korea strains may have migrated from European countries. BQCV-JL1 strain was different from the other 6 strains in dividing the nucleotides positions of QRF, which vqs because of the gene mutation.
AuthorsQian Yang, Jian Zhang, Zhanyun Song, Yan Zheng, Xianghui Wang, Jiachen Sui, Zhenguo Wang, Jun Mou
JournalBing du xue bao = Chinese journal of virology (Bing Du Xue Bao) Vol. 31 Issue 2 Pg. 114-23 (Mar 2015) ISSN: 1000-8721 [Print] China
PMID26164935 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Viral Proteins
Topics
  • Amino Acid Sequence
  • Animals
  • Bees
  • China
  • Genome, Viral
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • RNA Viruses (classification, genetics, isolation & purification)
  • Viral Proteins (genetics)

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