Ginsenoside Rb1 Treatment Attenuates Pulmonary Inflammatory Cytokine Release and Tissue Injury following Intestinal Ischemia Reperfusion Injury in Mice.

Abstract

Objective. Intestinal ischemia reperfusion (II/R) injury plays a critical role in remote organ dysfunction, such as lung injury, which is associated with nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. In the present study, we tested whether ginsenoside Rb1 attenuated II/R induced lung injury by Nrf2/HO-1 pathway. Methods. II/R injury was induced in male C57BL/6J mice by 45 min of superior mesenteric artery (SMA) occlusion followed by 2 hours of reperfusion. Ginsenoside Rb1 was administrated prior to reperfusion with or without ATRA (all-transretinoic acid, the inhibitor of Nrf2/ARE signaling pathway) administration before II/R. Results. II/R induced lung histological injury, which is accompanied with increased levels of malondialdehyde (MDA), interleukin- (IL-) 6, and tumor necrosis factor- (TNF-) α but decreased levels of superoxide dismutase (SOD) and IL-10 in the lung tissues. Ginsenoside Rb1 reduced lung histological injury and the levels of TNF-α and MDA, as well as wet/dry weight ratio. Interestingly, the increased Nrf2 and HO-1 expression induced by II/R in the lung tissues was promoted by ginsenoside Rb1 treatment. All these changes could be inhibited or prevented by ATRA. Conclusion. Ginsenoside Rb1 is capable of ameliorating II/R induced lung injuries by activating Nrf2/HO-1 pathway.

• Authors: Ying Jiang, Zhen Zhou, Qing-tao Meng, Qian Sun, Wating Su, Shaoqing Lei, Zhengyuan Xia, Zhong-yuan Xia
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2015 Pg. 843721 ( 2015) ISSN: 1942-0994 [Electronic] United States
• PMID: 26161243 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ginsenosides
• Interleukin-6
• NF-E2-Related Factor 2
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Malondialdehyde
• Tretinoin
• ginsenoside Rb1
• Heme Oxygenase-1
• Superoxide Dismutase

Topics

• Animals
• Ginsenosides (pharmacology)
• Heme Oxygenase-1 (metabolism)
• Interleukin-10 (metabolism)
• Interleukin-6 (metabolism)
• Intestines (pathology)
• Lung (metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Mice
• Mice, Inbred C57BL
• NF-E2-Related Factor 2 (antagonists & inhibitors, metabolism)
• Reperfusion Injury (metabolism, pathology)
• Signal Transduction (drug effects)
• Superoxide Dismutase (metabolism)
• Tretinoin (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)