Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria.

Abstract

BACKGROUND/AIMS:

We previously showed that urine and serum Angiopoietin-2 (Ang-2) levels increased significantly with the degree of albuminuria in diabetes patients, but the reasons remain unclear. Consequently we aimed to determine whether there was an association between Ang-2, inflammatory cytokines (TNF-α and IL-18) and reactive oxygen species (8-OHdG and SOD) in type 2 diabetes patients with albuminuria.

METHODS:

This retrospective study evaluated 113 patients with type 2 diabetes and normoalbuminuria, microalbuminuria, or macroalbuminuria and 30 healthy controls. Serum and urine TNF-α, IL-18 and 8-OHdG levels were measured by ELISA. Superoxide dismutase (SOD) activity was determined by spectrophotometry.

RESULTS:

Serum and urine TNF-α, IL-18 and 8-OHdG levels increased significantly with the degree of albuminuria, and were positively correlated with increased Ang-2. In contrast, SOD activity decreased with the degree of albuminuria and was negatively correlated with Ang-2. Multivariable linear regression analysis revealed that serum Ang-2 level was independently associated with serum levels of TNF-α (P<0.001), 8-OHdG (P=0.001), and IL-18 (P=0.003). Urinary Ang-2 level was independently associated with urinary TNF-α (P<0.001) and 8-OHdG (P=0.004) levels.

CONCLUSION:

TNF-α and 8-OHdG are associated with elevated urinary Angiopoietin-2 levels in type 2 diabetic patients with albuminuria.

• Authors: Fang-Fang He, Hui-Qing Li, Qing-Xian Huang, Qiu-Yuan Wang, Hua-Jun Jiang, Shan Chen, Hua Su, Chun Zhang, Yu-Mei Wang
• Journal: Kidney & blood pressure research (Kidney Blood Press Res) Vol. 40 Issue 4 Pg. 355-65 ( 2015) ISSN: 1423-0143 [Electronic] Switzerland
• PMID: 26160410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 S. Karger AG, Basel.

Chemical References

• ANGPT2 protein, human
• Angiopoietin-2
• Interleukin-18
• Reactive Oxygen Species
• SOD1 protein, human
• Tumor Necrosis Factor-alpha
• 8-Hydroxy-2'-Deoxyguanosine
• Superoxide Dismutase
• Superoxide Dismutase-1
• NADPH Oxidase 4
• NADPH Oxidases
• NOX4 protein, human
• Deoxyguanosine

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Adult
• Albuminuria (blood)
• Angiopoietin-2 (urine)
• Cross-Sectional Studies
• Deoxyguanosine (analogs & derivatives, metabolism)
• Diabetes Mellitus, Type 2 (blood)
• Female
• Humans
• Interleukin-18 (metabolism)
• Kidney Glomerulus (metabolism)
• Male
• Middle Aged
• NADPH Oxidase 4
• NADPH Oxidases (metabolism)
• Reactive Oxygen Species (metabolism)
• Retrospective Studies
• Superoxide Dismutase (blood, metabolism)
• Superoxide Dismutase-1
• Tumor Necrosis Factor-alpha (metabolism)