Abstract |
The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo- blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell culture model. In this study, we demonstrated that Tulane virus (TV), a cultivable primate calicivirus, also recognizes SAs in addition to the previously known TV-HBGA interactions. Evidence supporting this discovery includes that TV virions bound synthetic sialoglycoconjugates (SGCs) and that treatment of TV permissive LLC-MK2 cells with either neuraminidases or SA-binding lectins inhibited TV infectivity. In addition, we found that Maackia amurensis leukoagglutinin (MAL), a lectin that recognizes the α-2,3 linked SAs, bound LLC-MK2 cells, as well as TV, by which MAL promoted TV infectivity in cell culture. Our findings further highlight TV as a valuable surrogate for huNoVs, particularly in studying virus-host interactions that may involve two host carbohydrate receptors or co-receptors for infection.
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Authors | Ming Tan, Chao Wei, Pengwei Huang, Qiang Fan, Christina Quigley, Ming Xia, Hao Fang, Xufu Zhang, Weiming Zhong, John S Klassen, Xi Jiang |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 11784
(Jul 06 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26146020
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Group Antigens
- Phytohemagglutinins
- RNA, Viral
- Receptors, Cell Surface
- Sialic Acids
- leukoagglutinins, plants
- Neuraminidase
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Topics |
- Animals
- Blood Group Antigens
(genetics, metabolism)
- Caliciviridae
(isolation & purification, physiology)
- Cell Line
- Feces
(virology)
- Host-Pathogen Interactions
- Humans
- Maackia
(metabolism)
- Macaca mulatta
(virology)
- Microscopy, Fluorescence
- Neuraminidase
(metabolism)
- Norovirus
(physiology)
- Phytohemagglutinins
(chemistry, metabolism)
- RNA, Viral
(analysis)
- Real-Time Polymerase Chain Reaction
- Receptors, Cell Surface
(chemistry, metabolism)
- Sialic Acids
(chemistry, metabolism)
- Virus Internalization
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