A CD44 specific peptide developed by phage display for targeting gastric cancer.

Abstract

OBJECTIVE:

To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity.

RESULTS:

A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most highly selective peptide, which termed RP-1. Immunofluorescence and flow cytometry analysis indicated that the consensus peptide RP-1 could bind to CD44-positive gastric cancer cells with mean fluorescence intensities significantly higher than that of CD44-negative cells. CD44 knockdown led to decreased binding activity of RP-1 to the same cell line. Tissue array technique was used to identify the relationship (r = 0.556) between peptide binding and CD44 detection on gastric cancer tissues. Further, the hyaluronan-binding domain of CD44 was docked with RP-1 using computer modeling/docking approaches, revealing a RP-1/CD44 interaction with geometrical and energy match (-8.6 kcal/mol).

CONCLUSIONS:

The RP-1 peptide we screened exhibits affinity and specificity to CD44 on cells and has the potential to be used as a candidate probe for gastric cancer cell targeting.

• Authors: Dan Zhang, Huan Jia, Yan Wang, Wei-Ming Li, Ying-Chun Hou, Shi-Wei Yin, Thomas D Wang, Shui-Xiang He, Shao-Ying Lu
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 37 Issue 11 Pg. 2311-20 (Nov 2015) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 26140900 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD44 protein, human
• Hyaluronan Receptors
• Peptide Library
• Peptides

Topics

• Cell Line, Tumor
• Humans
• Hyaluronan Receptors (chemistry, metabolism)
• Immunohistochemistry
• Molecular Docking Simulation
• Peptide Library
• Peptides (chemistry, genetics, metabolism)
• Stomach Neoplasms (chemistry, metabolism)