Contrast Agent Mass Spectrometry Imaging Reveals Tumor Heterogeneity.

Abstract

Mapping intratumoral heterogeneity such as vasculature and margins is important during intraoperative applications. Desorption electrospray ionization mass spectrometry (DESI-MS) has demonstrated potential for intraoperative tumor imaging using validated MS profiles. The clinical translation of DESI-MS into a universal label-free imaging technique thus requires access to MS profiles characteristic to tumors and healthy tissues. Here, we developed contrast agent mass spectrometry imaging (CA-MSI) that utilizes a magnetic resonance imaging (MRI) contrast agent targeted to disease sites, as a label, to reveal tumor heterogeneity in the absence of known MS profiles. Human breast cancer tumors grown in mice were subjected to CA-MSI using Gadoteridol revealing tumor margins and vasculature from the localization of [Gadoteridol+K](+) and [Gadoteridol+Na](+) adducts, respectively. The localization of the [Gadoteridol+K](+) adduct as revealed through DESI-MS complements the in vivo MRI results. DESI-MS imaging is therefore possible for tumors for which no characteristic MS profiles are established. Further DESI-MS imaging of the flux of the contrast agent through mouse kidneys was performed indicating secretion of the intact label.

• Authors: Alessandra Tata, Jinzi Zheng, Howard J Ginsberg, David A Jaffray, Demian R Ifa, Arash Zarrine-Afsar
• Journal: Analytical chemistry (Anal Chem) Vol. 87 Issue 15 Pg. 7683-9 (Aug 04 2015) ISSN: 1520-6882 [Electronic] United States
• PMID: 26138213 (Publication Type: Journal Article)

Chemical References

• Contrast Media

Topics

• Animals
• Breast Neoplasms (diagnosis, pathology)
• Contrast Media
• Female
• Humans
• Magnetic Resonance Imaging
• Mice
• Spectrometry, Mass, Electrospray Ionization