Rapid and simple colorimetric detection of Escherichia coli O157:H7 in apple juice using a novel recombinant bacteriophage-based Method.

Abstract

In this study, a bacteriophage-based method for the colorimetric detection of E. coli O157:H7 in apple juice was investigated. Firstly, a gene encoding Cytochrome c Peroxidase (CCP) chromogenic enzyme was inserted into a wild type PP01 phage genome to construct the recombinant PP01ccp phage that was used in the production of the chromogenic enzyme through specific infection into E. coli O157:H7. The method was then examined in the colorimetric detection of E. coli O157:H7 in broth, and the appearance of E. coli O157:H7 in broth was confirmed by the color change after a few minutes of the enzyme assay. Secondly, the method was investigated in the colorimetric detection of E. coli O157:H7 in apple juice. A low E. coli O157:H7 concentration as 1 CFU mL(-1) was detected in 15 h that was in a shorter time than in previous bioluminescence phage-based methods. Moreover, the method is much simpler compared to other previous phage-based methods since it enables detection without the need for expensive apparatus.

• Authors: Hoang A Hoang, Le T Dien
• Journal: Biocontrol science (Biocontrol Sci) Vol. 20 Issue 2 Pg. 99-103 ( 2015) ISSN: 1884-0205 [Electronic] Japan
• PMID: 26133507 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Cytochrome-c Peroxidase

Topics

• Bacteriological Techniques (methods)
• Bacteriophages (genetics, growth & development)
• Carbonated Beverages (microbiology)
• Colorimetry (methods)
• Cytochrome-c Peroxidase (analysis, genetics)
• Escherichia coli O157 (isolation & purification, virology)
• Food Microbiology (methods)
• Genes, Reporter
• Genome, Viral
• Malus
• Recombination, Genetic
• Sensitivity and Specificity
• Time Factors