Quantitative analysis of differential protein expression in cervical carcinoma cells after zeylenone treatment by stable isotope labeling with amino acids in cell culture.

Abstract

Cervical carcinoma is a malignant tumor that poses a serious threat to women's health and survival. Approximately 10-25% of cervical cancers are adenocarcinomas (ACs). AC has high rates of recurrence and mortality, while there is no effective treatment for now. Zeylenone (Zey), which is isolated from an ethanol extract of the leaves of Uvaria grandiflora Roxb. of the family Annonaceae, has shown potent inhibitory activity against various tumor cells, including cervical carcinoma cells. To gain insight into the molecular mechanism underlying the effect of Zey on AC, we quantified protein expression changes in AC cells treated with Zey. We used stable isotope labeling with amino acids in cell culture (SILAC) in combination with high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) and bioinformatics analysis to compare protein expression profiles in HeLa cells before and after Zey treatment. Of 1805 differentially expressed proteins identified, 229 were screened as key protein molecules and classified into nine categories. Profiling of differentially-expressed proteins contributed to our understanding of the molecular mechanism by which Zey induces HeLa cell apoptosis. Using this method, candidate targets can be identified for developing new drugs against cervical carcinoma.

• Authors: Leilei Zhang, Jing Jin, Lijing Zhang, Ruifeng Hu, Li Gao, Xiaowei Huo, Dongyu Liu, Xiaoling Ma, Canhong Wang, Jiayuan Han, Liyong Li, Xiaobo Sun, Li Cao
• Journal: Journal of proteomics (J Proteomics) Vol. 126 Pg. 279-87 (Aug 03 2015) ISSN: 1876-7737 [Electronic] Netherlands
• PMID: 26130516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Amino Acids
• Cyclohexanes
• Dioxanes
• Neoplasm Proteins
• zeylenone

Topics

• Amino Acids (chemistry, metabolism)
• Cyclohexanes (pharmacology)
• Dioxanes (pharmacology)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• HeLa Cells
• Humans
• Isotope Labeling (methods)
• Neoplasm Proteins (biosynthesis)
• Proteomics (methods)
• Uterine Cervical Neoplasms (metabolism)