Anticancer effect of tectochrysin in colon cancer cell via suppression of NF-kappaB activity and enhancement of death receptor expression.

Abstract

BACKGROUND:

Flavonoids are a diverse family of natural phenolic compounds commonly found in fruits and vegetables. Epidemiologic studies showed that flavonoids also reduce the risk of colon cancer. Tectochrysin is one of the major flavonoids of Alpinia oxyphylla Miquel. However, the anti-cancer effects and the molecular mechanisms of tectochrysin in colon cancer cells have not yet been reported. We investigated whether tectochrysin could inhibit colon cancer cell growth at 1, 5, 10 μg/ml. In in vivo study, we injected a tectochrysin treatment dose of 5 mg/kg to each mouse.

RESULTS:

Tectochrysin suppressed the growth of SW480 and HCT116 human colon cancer cells. The expression of DR3, DR4 and Fas were significantly increased, and pro-apoptotic proteins were also increased. Tectochrysin treatment also inhibited activity of NF-κB. A docking model indicated that tectochrysin binds directly to the p50 unit. In in vivo, tumor weights and volumes in mice were reduced when treated with tectochrysin. Tectochrysin leads to apoptotic cell death in colon cancer cells through activation of death receptors expression via the inhibition of NF-κB.

CONCLUSIONS:

Tectochrysin can be a useful agent for the treatment of colon cancer cell growth as well as an adjuvant agent for chemo-resistant cancer cells growth.

• Authors: Mi Hee Park, Ji Eun Hong, Eun Sook Park, Hee Sung Yoon, Doo Won Seo, Byung Kook Hyun, Sang-Bae Han, Young Won Ham, Bang Yeon Hwang, Jin Tae Hong
• Journal: Molecular cancer (Mol Cancer) Vol. 14 Pg. 124 (Jun 30 2015) ISSN: 1476-4598 [Electronic] England
• PMID: 26123287 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Apoptosis Regulatory Proteins
• FAS protein, human
• Flavonoids
• NF-kappa B
• RNA, Small Interfering
• Receptors, Death Domain
• TNF-Related Apoptosis-Inducing Ligand
• fas Receptor
• tectochrysin
• Caspase 3

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Caspase 3 (metabolism)
• Cell Proliferation (drug effects)
• Colonic Neoplasms (metabolism, pathology)
• Drug Resistance, Neoplasm (drug effects)
• Flavonoids (chemistry, pharmacology)
• HCT116 Cells
• Humans
• Male
• Mice, Inbred BALB C
• Mice, Nude
• NF-kappa B (metabolism)
• RNA, Small Interfering (metabolism)
• Receptors, Death Domain (metabolism)
• TNF-Related Apoptosis-Inducing Ligand (pharmacology)
• Xenograft Model Antitumor Assays
• fas Receptor (metabolism)