Effects of Selenium Supplementation on Spontaneous Autoimmune Thyroiditis in NOD.H-2h4 Mice.

Abstract

BACKGROUND:

Recent clinical studies have demonstrated the suppressive effect of selenium (Se) treatment on serum thyroid-specific antibody titers in patients with autoimmune thyroiditis (AIT), but the mechanism underlying this process is not clear. The aim of the present study was to investigate the effects of selenium on the incidence and severity of AIT, titers of thyroid autoantibodies, and selenoprotein expression in thyroid in a spontaneous autoimmune thyroiditis (SAT) model.

METHODS:

NOD.H-2(h4) mice at four weeks of age were randomly divided into control, iodine supplement (SAT), and selenium supplement groups (SAT+Se). Mice were given 0.005% sodium iodide water for eight weeks to induce SAT and then 0.3 mg/L sodium selenite in drinking water for 8 weeks and 16 weeks. The severity of lymphocytic infiltration in the thyroid, serum thyroglobulin antibody (TgAb) titers, serum selenium concentration, expression of glutathione peroxidase-1 (GPx1), thioredoxin reductase-1 (Txnrd1), and peroxiredoxin 5 were measured.

RESULTS:

Serum selenium concentration significantly increased after selenium supplementation. Serum TgAb levels were significantly lower in the selenium group compared with the SAT group (p<0.05). The prevalence of thyroiditis and the degree of infiltration of lymphocytes decreased gradually over time in the group provided with selenium supplementation. The expression of GPx1 and Txnrd1 by Western blotting were found to be significantly higher in the SAT+Se group than in other groups (p<0.05).

CONCLUSIONS:

These results indicate that selenium treatment can increase the function of antioxidation by upregulating the expression of selenoproteins in the thyroid and have an inhibitory effect on TgAb titers, which may have an impact on AIT.

• Authors: Weiwei Wang, Haibo Xue, Yushu Li, Xin Hou, Chenling Fan, Hong Wang, Hongmei Zhang, Zhongyan Shan, Weiping Teng
• Journal: Thyroid : official journal of the American Thyroid Association (Thyroid) Vol. 25 Issue 10 Pg. 1137-44 (Oct 2015) ISSN: 1557-9077 [Electronic] United States
• PMID: 26121912 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Thyroglobulin
• Peroxiredoxins
• Iodide Peroxidase
• Glutathione Peroxidase
• Thioredoxin Reductase 1
• Sodium Iodide
• Selenium
• Sodium Selenite
• Glutathione Peroxidase GPX1
• Gpx1 protein, mouse

Topics

• Animals
• Autoantibodies (blood)
• Dietary Supplements
• Disease Models, Animal
• Glutathione Peroxidase (metabolism)
• Iodide Peroxidase (metabolism)
• Mice
• Mice, Inbred NOD
• Peroxiredoxins (metabolism)
• Selenium (blood)
• Sodium Iodide
• Sodium Selenite (therapeutic use)
• Thioredoxin Reductase 1 (metabolism)
• Thyroglobulin (immunology)
• Thyroid Gland (immunology)
• Thyroiditis, Autoimmune (blood, chemically induced, drug therapy, immunology)
• Glutathione Peroxidase GPX1