HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Simultaneous determination of leucine, isoleucine and valine in Beagle dog plasma by HPLC-MS/MS and its application to a pharmacokinetic study.

Abstract
Leucine (Leu), isoleucine (Ile) and valine (Val) are three branched-chain amino acids (BCAAs), which have been widely used as dietary supplements for professional athletes and patients with liver failure or catabolic diseases. To date, no pharmacokinetic studies of BCAAs in vivo useful for the assessment of clinical effect following daily intake has been reported. Thus in this study, an HPLC-MS/MS method for simultaneous determination of Leu, Ile and Val in Beagle dog plasma using homoarginine as the internal standard was developed and validated in terms of specificity, linearity, precision, accuracy, and stability. This assay method was then applied to a pharmacokinetic study of BCAAs in dogs following oral administration of 0.25 g/kg and 0.50 g/kg BCAAs. The HPLC-MS/MS method was found to be sensitive and reproducible for quantification of BCAAs in dog plasma and successfully applied to the pharmacokinetic study. All these BCAAs were well absorbed with a substantial increase in the plasma concentration after a baseline modification. No statistical significance was identified in different gender group and no drug accumulation was observed following multiple doses.
AuthorsTing Wang, Huiru Xie, Xu Chen, Xuehua Jiang, Ling Wang
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 114 Pg. 426-32 (Oct 10 2015) ISSN: 1873-264X [Electronic] England
PMID26119505 (Publication Type: Journal Article, Validation Study)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Isoleucine
  • Leucine
  • Valine
Topics
  • Administration, Oral
  • Animals
  • Calibration
  • Chromatography, High Pressure Liquid (methods)
  • Dogs
  • Female
  • Isoleucine (blood, pharmacokinetics)
  • Leucine (blood, pharmacokinetics)
  • Male
  • Plasma (chemistry)
  • Quality Control
  • Reproducibility of Results
  • Tandem Mass Spectrometry (methods)
  • Valine (blood, pharmacokinetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: