Fatty acid synthase is a metabolic oncogene targetable in malignant peripheral nerve sheath tumors.

Abstract

BACKGROUND:

Malignant peripheral nerve sheath tumors (MPNSTs) are soft tissue sarcomas with minimal therapeutic opportunities. We observed that lipid droplets (LDs) accumulate in human MPNST cell lines and in primary human tumor samples. The goal of this study was to investigate the relevance of lipid metabolism to MPNST survival and as a possible therapeutic target.

METHODS:

Based on preliminary findings that MPNSTs accumulate LDs, we hypothesized that a deregulated lipid metabolism supports MPNST cell survival/proliferation rate. To test this, we examined respiration, role of fatty acid oxidation (FAO), and the enzyme fatty acid synthase involved in de novo fatty acid synthesis in MPNSTs using both genetic and pharmacological tools.

RESULTS:

We demonstrate that LDs accumulate in MPNST cell lines, primary human and mouse MPNST tumors, and neural crest cells. LDs from MPNST cells disappear on lipid deprivation, indicating that LDs can be oxidized as a source of energy. Inhibition of FAO decreased oxygen consumption and reduced MPNST survival, indicating that MPNST cells likely metabolize LDs through active FAO. FAO inhibition reduced oxygen consumption and survival even in the absence of exogenous lipids, indicating that lipids synthesized de novo can also be oxidized. Consequently, inhibition of de novo fatty acid synthesis, which is overexpressed in human MPNST cell lines, effectively reduced MPNST survival and delayed induction of tumor growth in vivo.

CONCLUSION:

Our results show that MPNSTs depend on lipid metabolic pathways and suggest that disrupting lipid metabolism could be a potential new strategy for the development of MPNST therapeutics.

• Authors: Ami V Patel, Gunnar Johansson, Melissa C Colbert, Biplab Dasgupta, Nancy Ratner
• Journal: Neuro-oncology (Neuro Oncol) Vol. 17 Issue 12 Pg. 1599-608 (Dec 2015) ISSN: 1523-5866 [Electronic] England
• PMID: 26116612 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• 4-methylene-2-octyl-5-oxofuran-3-carboxylic acid
• Fatty Acid Synthases
• 4-Butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives, pharmacology)
• Animals
• Cell Growth Processes (drug effects)
• Cell Line, Tumor
• Cell Movement
• Cell Survival (drug effects)
• Fatty Acid Synthases (antagonists & inhibitors, metabolism)
• Humans
• Lipid Droplets (metabolism)
• Mice
• Mice, Inbred C57BL
• Neural Crest (metabolism)
• Neurilemmoma (metabolism)
• Schwann Cells (metabolism)
• Xenograft Model Antitumor Assays