Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS: RESULTS: The MTT assay demonstrated that 50, 500, and 1,500 μg/mL of gentiopicroside exhibited no significant toxicity to chondrocytes (P>0.05) after 24h. Using immunohistochemistry, ELISA, RT-PCR, Western blot method to explore the protective effect and mechanism of gentiopicroside on chondrocytes induced by IL-1β. The results showed some pathways of IL-1β signal transduction were inhibited by gentiopicroside in rat chondrocytes: p38, ERK and JNK. Meanwhile, gentiopicroside showed inhibition in the IL-1β-induced release of MMPs while increasing Collagen type II expression. CONCLUSIONS: The current study demonstrated that gentiopicroside exhibited a potent protective effect on IL-1β induced inflammation response in rat articular chondrocyte. Thus, gentiopicroside could be a potential therapeutic strategy for treatment of OA.
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Authors | Lei Zhao, Juan Ye, Guo-Tai Wu, Xue-Jing Peng, Peng-Fei Xia, Yuan Ren |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 172
Pg. 100-7
(Aug 22 2015)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 26116164
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Collagen Type II
- Interleukin-1beta
- Iridoid Glucosides
- gentiopicroside
- Cyclooxygenase 2
- Mitogen-Activated Protein Kinases
- Collagenases
- Dinoprostone
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Topics |
- Animals
- Anti-Inflammatory Agents
(isolation & purification, pharmacology, therapeutic use)
- Cell Survival
(drug effects)
- Cells, Cultured
- Chondrocytes
(drug effects, metabolism)
- Collagen Type II
(metabolism)
- Collagenases
(biosynthesis)
- Cyclooxygenase 2
(biosynthesis)
- Dinoprostone
(metabolism)
- Gene Expression
(drug effects)
- Inflammation
(drug therapy, immunology, prevention & control)
- Interleukin-1beta
(antagonists & inhibitors, immunology)
- Iridoid Glucosides
(isolation & purification, pharmacology, therapeutic use)
- Mitogen-Activated Protein Kinases
(metabolism)
- Rats
- Signal Transduction
(drug effects)
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