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Smart Probe for Tracing Cancer Therapy: Selective Cancer Cell Detection, Image-Guided Ablation, and Prediction of Therapeutic Response In Situ.

Abstract
Integrated diagnosis and therapy systems that can offer traceable cancer therapy are in high demand for personalized medicine. Herein, a pH-responsive polymeric probe containing tetraphenylsilole (TPS) with aggregation-induced emission characteristics and pheophorbide A (PheA) photosensitizer (PS) with aggregation-caused quenching property for tracing the whole process of cancer therapy is reported. At physiological conditions (pH 7.4), the probe self-assembles into nanoparticles (NPs), which show weak fluorescence of PheA with low phototoxicity, but strong green fluorescence from TPS for probe self-tracking. Upon uptake by cancer cells and entrapment in lysosomes (pH 5.0), the NPs disassemble to yield weak emission of TPS but strong red fluorescence of PheA with restored phototoxicity for PS activation monitoring. Upon light irradiation, the generated reactive oxygen species can cause lysosomal disruption to trigger cell apoptosis. Meanwhile, the probe leaks to the cytoplasm (pH 7.2), where the TPS fluorescence is restored for in situ visualization of the therapeutic response. The probe design thus represents a novel strategy for traceable cancer therapy.
AuthorsYouyong Yuan, Ryan T K Kwok, Ben Zhong Tang, Bin Liu
JournalSmall (Weinheim an der Bergstrasse, Germany) (Small) Vol. 11 Issue 36 Pg. 4682-90 (Sep 2015) ISSN: 1613-6829 [Electronic] Germany
PMID26113312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chemical References
  • Fluorescent Dyes
  • Photosensitizing Agents
  • Polymers
  • Reactive Oxygen Species
  • Chlorophyll
  • Green Fluorescent Proteins
  • Polylysine
  • pheophorbide a
Topics
  • Apoptosis
  • Cell Line, Tumor
  • Chlorophyll (analogs & derivatives, chemistry)
  • Cytoplasm (metabolism)
  • Cytosol (metabolism)
  • Fluorescent Dyes (chemistry)
  • Green Fluorescent Proteins (chemistry)
  • HEK293 Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Lysosomes (metabolism)
  • MCF-7 Cells
  • Microscopy, Fluorescence
  • Nanoparticles (chemistry)
  • Neoplasms (metabolism, therapy)
  • Photosensitizing Agents (chemistry)
  • Polylysine (chemistry)
  • Polymers (chemistry)
  • Precision Medicine
  • Reactive Oxygen Species (metabolism)

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