Abstract | BACKGROUND: METHODS: Female F344 rats (n = 60) were divided among six experimental groups: (1) RT, single dose of 21 Gy to the right hemithorax; (2) RT + 5 mg/kg bSOD; (3) RT + 15 mg/kg bSOD; (4) No RT; (5) sham RT + 5 mg/kg bSOD; and (6) sham RT + 15 mg/kg bSOD. A single subcutaneous injection of bSOD (5 or 15 mg/kg) was administered 24 h post-radiation. The effects of bSOD on radiation-induced lung injury were assessed by measurement of body weight, breathing frequency, and histopathological changes. Immunohistochemistry was used to evaluate oxidative stress (8-OHdG(+), NOX4(+), nitrotyrosine(+), and 4HNE(+) cells), macrophage activation (ED1(+)), and expression of profibrotic transforming growth factor-β or TGF-β in irradiated tissue. RESULTS: Radiation led to an increase in all the evaluated parameters. Treatment with 15 mg/kg bSOD significantly decreased levels of all the evaluated parameters including tissue damage and breathing frequency starting 6 weeks post-radiation. Animals treated with 5 mg/kg bSOD trended toward a suppression of radiation-induced lung damage but did not reach statistical significance. CONCLUSIONS:
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Authors | Vlado Antonic, Zahid N Rabbani, Isabel L Jackson, Zeljko Vujaskovic |
Journal | Free radical research
(Free Radic Res)
Vol. 49
Issue 10
Pg. 1259-1268
(Oct 2015)
ISSN: 1029-2470 [Electronic] England |
PMID | 26110460
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antioxidants
- Metalloproteins
- Radiation-Protective Agents
- Reactive Nitrogen Species
- Reactive Oxygen Species
- Collagen
- Superoxide Dismutase
- orgotein
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Topics |
- Animals
- Antioxidants
(administration & dosage, pharmacology, therapeutic use)
- Body Weight
(drug effects, radiation effects)
- Cattle
- Collagen
(analysis)
- Female
- Fibrosis
- Injections, Subcutaneous
- Lung
(chemistry, drug effects, physiopathology, radiation effects)
- Macrophage Activation
(drug effects, radiation effects)
- Metalloproteins
(administration & dosage, pharmacology, therapeutic use)
- Radiation Injuries, Experimental
(prevention & control)
- Radiation Pneumonitis
(pathology, prevention & control)
- Radiation-Protective Agents
(administration & dosage, pharmacology, therapeutic use)
- Rats
- Rats, Inbred F344
- Reactive Nitrogen Species
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Respiratory Rate
(drug effects, radiation effects)
- Superoxide Dismutase
(administration & dosage, pharmacology, therapeutic use)
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