Immunohistochemical Expression and Prognostic Significance of CD97 and its Ligand DAF in Human Cervical Squamous Cell Carcinoma.

Abstract

Accumulating evidences had demonstrated that the CD97, a member of the epidermal growth factor 7-transmembrane family, and its cellular ligand decay accelerating factor (DAF) both play important roles in tumor dedifferentiation, migration, invasiveness, and metastasis. However, the roles of CD97 and DAF in human cervical squamous cell carcinoma (CSCC) have not been investigated. The purpose of this study was to observe the expression profile of CD97 and DAF in CSCC and evaluate their clinical significance. Immunohistochemistry was used to investigate the expression of CD97 and DAF proteins in 97 patients with CSCC and 53 patients with cervical intraepithelial neoplasia, a precursor lesion of CSCC. CD97 and DAF were absent or only weakly expressed in the normal epithelium of the cervix but were present in 83.5% (81/97) and 90.7% (88/97) of CSCC samples, respectively. Overexpression of CD97 was significantly associated with a high International Federation of Gynecology and Obstetrics stage (P=0.010) and lymph node metastasis (P=0.026). The majority of CSCCs, irrespective of staging/grading classification, displayed strong DAF immunostaining. Kaplan-Meier survival analysis revealed that overexpression of CD97 was associated with a worse prognosis. Multivariate analyses showed that the International Federation of Gynecology and Obstetrics stage (P=0.000), lymph node metastasis (P=0.004), and CD97 expression (P=0.040) were independent risk factors for overall survival. The present study suggested that the expressions of CD97 and DAF were both upregulated in CSCC. The expression level of CD97 in CSCC was associated with the severity of the tumor. Furthermore, CD97 might be an independent poor prognostic factor for CSCC patients.

• Authors: Ying He, Wei Wang, Lian Xu, Li Li, Juan Liu, Min Feng, Hong Bu
• Journal: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (Int J Gynecol Pathol) Vol. 34 Issue 5 Pg. 473-9 (Sep 2015) ISSN: 1538-7151 [Electronic] United States
• PMID: 26107567 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ADGRE5 protein, human
• Antigens, CD
• CD55 Antigens
• Receptors, G-Protein-Coupled

Topics

• Adult
• Antigens, CD (analysis)
• CD55 Antigens (analysis)
• Carcinoma, Squamous Cell (chemistry, mortality, pathology)
• Female
• Humans
• Immunohistochemistry
• Lymphatic Metastasis (pathology)
• Middle Aged
• Neoplasm Staging
• Prognosis
• Receptors, G-Protein-Coupled
• Risk Factors
• Survival Rate
• Uterine Cervical Neoplasms (chemistry, mortality, pathology)
• Uterine Cervical Dysplasia (chemistry, pathology)