Abstract |
Osteosarcoma is the most common primary bone tumor in humans, especially in childhood. However, the genetic etiology for its pathogenesis remains elusive. It is known that microRNAs ( miRNAs) are involved in the development of tumor progression. Here we show that microRNA-9 (miR-9) is a potential oncogene upregulated in osteosarcoma cells. Knockdown of miR-9 in osteosarcoma resulted in suppressed colony formation and cell proliferation. Further study identified GCIP, a Grap2 and cyclin D interacting protein, as a direct target of miR- 9. In addition, GCIP overexpression activated retinoblastoma 1 (Rb) and suppressed E2F transcriptional target expression in osteosarcoma cells. Moreover, GCIP depletion reversed miR-9 knockdown induced colony formation and cell proliferation suppression. In sum, these results highlight the importance of miR-9 as an oncogene in regulating the proliferation of osteosarcoma by directly targeting GCIP and may provide new insights into the pathogenesis of osteosarcoma.
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Authors | Shao-Wen Zhu, Jian-Peng Li, Xin-Long Ma, Jian-Xiong Ma, Yang Yang, Yang Chen, Wei Liu |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 16
Issue 11
Pg. 4509-13
( 2015)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 26107195
(Publication Type: Journal Article)
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Chemical References |
- CCNDBP1 protein, human
- MIRN92 microRNA, human
- MicroRNAs
- RNA, Messenger
- Transcription Factors
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Topics |
- Apoptosis
- Blotting, Western
- Bone Neoplasms
(genetics, metabolism, pathology)
- Cell Proliferation
- Colony-Forming Units Assay
- Gene Expression Regulation, Neoplastic
- Genes, Tumor Suppressor
- Humans
- MicroRNAs
(genetics)
- Osteosarcoma
(genetics, metabolism, pathology)
- RNA, Messenger
(genetics)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription Factors
(genetics, metabolism)
- Tumor Cells, Cultured
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