Inhibition of porcine reproductive and respiratory syndrome virus by Cecropin D in vitro.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to cause substantial economic losses to the pig industry worldwide. Although vaccines are commercially available for the control of PRRSV infection, no vaccination regimen has been proved sustained success in terms of generating a protective immune response. Therefore, the development of novel antivirals is urgently needed. Antimicrobial peptides display broad-spectrum antimicrobial activities against bacteria, fungi, and viruses and play an important role in host innate immune response. Here, we tested whether Cecropin D (CD) could inhibit PRRSV infection and replication in vitro. The inhibitory effect of CD occurred during viral attachment and the early period of viral entry into Marc-145 cells. CD also attenuated virus-induced apoptosis during the late phase of PRRSV infection and suppressed virus release in Marc-145 cells, which might contribute to the inhibition of PRRSV infection. Similar inhibitory effects on PRRSV infection were also found with CD treatment in porcine alveolar macrophages, the major target cell type of PRRSV infection in pigs in vivo. These findings suggest that CD has the potential to develop a new therapeutic agent against PRRSV infection.

• Authors: Xiaohong Liu, Chunhe Guo, Yumao Huang, Xiaoyu Zhang, Yaosheng Chen
• Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (Infect Genet Evol) Vol. 34 Pg. 7-16 (Aug 2015) ISSN: 1567-7257 [Electronic] Netherlands
• PMID: 26102162 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Antiviral Agents
• Insect Proteins
• Viral Proteins
• cecropin D protein, Hyalophora cecropia

Topics

• Animals
• Antiviral Agents (pharmacology)
• Apoptosis
• Cell Line
• Chlorocebus aethiops
• Insect Proteins (pharmacology)
• Microbial Sensitivity Tests
• Porcine respiratory and reproductive syndrome virus (drug effects, physiology)
• Protein Biosynthesis (drug effects)
• Sus scrofa
• Transcription, Genetic (drug effects)
• Viral Proteins (genetics, metabolism)
• Virus Attachment
• Virus Internalization
• Virus Release
• Virus Replication (drug effects)