Protective effect of cavidine on acetic acid-induced murine colitis via regulating antioxidant, cytokine profile and NF-κB signal transduction pathways.

Abstract

Ulcerative colitis is an inflammatory disorder characterized by neutrophils infiltration, oxidative stress, upregulation of pro-inflammatory mediators and cytokines. Cavidine possesses anti-inflammatory activity and has been used to treat various inflammatory diseases but its effect on ulcerative colitis has not been previously explored. The present study aims to evaluate the effect of cavidine on acetic acid-induced ulcerative colitis in mice. Colitis mice induced by intra-rectal acetic acid (5%, v/v) administration received cavidine (1, 5 and 10mg/kg, i.g) or sulfasalazine (500mg/kg, i.g) for seven consecutive days. After euthanized by cervical dislocation, colonic segments of mice were excised for clinical, macroscopic, biochemical and histopathological examinations. Results suggested treatment with cavidine significantly decreased mortality rate, body weight loss, disease activity index (DAI), wet colon weight, macroscopic and histological score when compared with that of acetic acid-induced controls. In addition, administration of cavidine effectively modulated expressions of MPO, GSH, SOD and MDA. Furthermore cavidine inhibited the level of TNF-α and IL-6 in the serum and colon tissue in response to the regulation of p65 NF-κB protein expression. All these results indicated cavidine exerts marked protective effect in experimental colitis, possibly by regulating the expression of oxygen metabolites, NF-κB and subsequent pro-inflammatory cytokines production.

• Authors: Xiaofeng Niu, Hailin Zhang, Weifeng Li, Yu Wang, Qingli Mu, Xiumei Wang, Zehong He, Huan Yao
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 239 Pg. 34-45 (Sep 05 2015) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 26102009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Ulcer Agents
• Antioxidants
• Berberine Alkaloids
• Cytokines
• Interleukin-6
• NF-kappa B
• Tumor Necrosis Factor-alpha
• cavidine
• Peroxidase
• Glutathione
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Anti-Ulcer Agents (pharmacology)
• Antioxidants (metabolism)
• Berberine Alkaloids (pharmacology)
• Colitis, Ulcerative (chemically induced, drug therapy, metabolism)
• Colon (drug effects, metabolism, pathology)
• Cytokines (metabolism)
• Disease Models, Animal
• Glutathione (metabolism)
• Interleukin-6 (blood)
• Male
• Mice
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Signal Transduction (drug effects)
• Tumor Necrosis Factor-alpha (blood, metabolism)