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Organotypic tissue culture investigation of homocysteine thiolactone cardiotoxic effect.

Abstract
Homocysteine thiolactone was demonstrated to inhibit the growth of 10-12-day-old chicken embryo cardiac tissue explants at 7 × 10⁻⁹ -1 × 10⁻³ M concentrations in a dose-dependent manner. The maximal cardiotoxic effect of homocysteine thiolactone was detected at 1 × 10⁻³ M, which corresponds to severe hyperhomocysteinemia. The results of experiments on culturing of cardiac tissue explants in the medium containing homocysteine thiolactone (1 × 10⁻³ M) and ouabain at concentrations regulating the signal-transducing (1 × 10⁻¹⁰ M) and pumping (1 × 10⁻⁸ M) functions of Na⁺,K⁺ -ATPase indicate that the cardiotoxic effect of homocysteine thiolactone is supposed to result from inhibition of the Na⁺,K⁺ -ATPase pumping function.
AuthorsEkaterina V Lopatina, A V Kipenko, V A Penniyaynen, N A Pasatetskaya, D Djuric, B V Krylov
JournalActa physiologica Hungarica (Acta Physiol Hung) Vol. 102 Issue 2 Pg. 137-42 (Jun 2015) ISSN: 0231-424X [Print] Hungary
PMID26100303 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Enzyme Inhibitors
  • Homocysteine
  • Ouabain
  • homocysteine thiolactone
  • Sodium-Potassium-Exchanging ATPase
Topics
  • Animals
  • Cell Proliferation (drug effects)
  • Chick Embryo
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors (toxicity)
  • Heart (drug effects, embryology, growth & development)
  • Homocysteine (analogs & derivatives, toxicity)
  • Myocytes, Cardiac (drug effects, metabolism, pathology)
  • Ouabain (pharmacology)
  • Signal Transduction (drug effects)
  • Sodium-Potassium-Exchanging ATPase (antagonists & inhibitors, metabolism)
  • Tissue Culture Techniques

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