Abstract |
In a previous paper, it was shown that cholera toxin B subunit (CTB) augments the production of protective antibodies to influenza virus when CTB is inoculated intranasally into Balb/c mice together with influenza HA vaccine. The present study was designed to determine whether the effectiveness of CTB as a potent adjuvant for nasal vaccination could be limited by pre-existing immunity to CTB. Mice were sensitized by intranasal inoculation of either 1 or 0.05 microgram of CTB 2, 4 and/or 6 weeks before nasal vaccination. They were then vaccinated by either a single inoculation of vaccine together with 1 microgram of CTB or a two-dose regimen, composed of a primary inoculation of vaccine together with 0.05 microgram of CTB and a subsequent inoculation of vaccine alone. Levels of nasal IgA antibodies to CTB increased with the increase of the dose of CTB and the frequency of CTB-inoculation. Pre-existing immunity to CTB, however, did not significantly reduce the levels of both nasal IgA and serum haemagglutination-inhibiting (HI) antibodies to influenza virus and did not change the ability of the vaccinated mice to resist viral challenge. These results suggest that a relatively low dose of CTB could be inoculated repeatedly into animals as an adjuvant for nasal vaccination.
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Authors | S Tamura, H Funato, T Nagamine, C Aizawa, T Kurata |
Journal | Vaccine
(Vaccine)
Vol. 7
Issue 6
Pg. 503-5
(Dec 1989)
ISSN: 0264-410X [Print] Netherlands |
PMID | 2609726
(Publication Type: Journal Article)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Viral
- Immunoglobulin A
- Influenza Vaccines
- Peptide Fragments
- Cholera Toxin
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Administration, Intranasal
- Animals
- Antibodies, Viral
(analysis)
- Cholera Toxin
(immunology, pharmacology)
- Female
- Immunoglobulin A
(analysis)
- Influenza Vaccines
(administration & dosage, immunology)
- Mice
- Mice, Inbred BALB C
- Peptide Fragments
(pharmacology)
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