Detection of Human α-L-Fucosidases by a Quinone Methide-Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection.

Abstract

α-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human α-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor.

• Authors: Manjula Nandakumar, Yu-Ling Hsu, Jason C-Y Lin, Chun Lo, Lee-Chiang Lo, Chun-Hung Lin
• Journal: Chembiochem : a European journal of chemical biology (Chembiochem) Vol. 16 Issue 11 Pg. 1555-9 (Jul 27 2015) ISSN: 1439-7633 [Electronic] Germany
• PMID: 26096673 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Indolequinones
• Lipopolysaccharides
• Molecular Probes
• quinone methide
• alpha-L-Fucosidase

Topics

• Cell Line, Tumor
• Epithelium (microbiology)
• Helicobacter pylori (physiology)
• Humans
• Indolequinones (metabolism)
• Lipopolysaccharides (metabolism)
• Molecular Probes (metabolism)
• Stomach (microbiology)
• alpha-L-Fucosidase (metabolism)