Polydatin: a new therapeutic agent against multiorgan dysfunction.

Abstract

BACKGROUND:

Polydatin (PD), a monocrystalline and polyphenolic drug isolated from a traditional Chinese herb (Polygonum cuspidatum), is protective against mitochondrial dysfunction and has been approved for clinical trials in the treatment of shock. However, whether the administration of PD has a therapeutic effect on multiple-organ dysfunction syndrome (MODS) requires investigation.

MATERIAL AND METHODS:

MODS was induced in Sprague-Dawley rats via hemorrhage and ligation and puncture of cecum-induced sepsis. The rats were divided into three groups as follows: MODS + PD, MODS + normal saline, and a control group (no treatment). Survival time, blood biochemical indexes, and histopathologic changes in various organs were evaluated; serum oxidative stress (advanced oxidative protein products [AOPPs]) and proinflammatory cytokines (tumor necrosis factor-α, interleukin 1β, and interleukin 6) were assayed using enzyme-linked immunosorbent assay. Apoptosis-related protein expression (B-cell lymphoma-2 [Bcl-2] and Bax) was assayed by immunohistochemical and Western blotting methods, whereas caspase-3 activity was assayed by spectrophotometry.

RESULTS:

PD improved organ function, prolonged survival time, and reduced MODS incidence and serum levels of AOPPs and proinflammatory cytokines. It also decreased Bax levels and caspase-3 activity and increased Bcl-2 levels in the kidney and liver.

CONCLUSIONS:

PD may serve as a potential therapeutic for MODS, as it suppresses oxidative stress, inhibits inflammatory response, attenuates apoptosis, and protects against mitochondrial dysfunction.

• Authors: Zhenhua Zeng, Zhongqing Chen, Tao Li, Junli Zhang, Youguang Gao, Siqi Xu, Shumin Cai, Ke-seng Zhao
• Journal: The Journal of surgical research (J Surg Res) Vol. 198 Issue 1 Pg. 192-9 (Sep 2015) ISSN: 1095-8673 [Electronic] United States
• PMID: 26095424 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Glucosides
• Proto-Oncogene Proteins c-bcl-2
• Stilbenes
• Casp3 protein, rat
• Caspase 3
• polydatin

Topics

• Animals
• Caspase 3 (metabolism)
• Cytokines (blood)
• Female
• Glucosides (therapeutic use)
• Multiple Organ Failure (drug therapy, immunology, mortality)
• Proto-Oncogene Proteins c-bcl-2 (analysis)
• Rats
• Rats, Sprague-Dawley
• Stilbenes (therapeutic use)