Abstract | BACKGROUND:
Thymosin α-1 (Tα1) exploits a specific action on lymphoid cells and is able to induce in peripheral blood mononuclear cells (PBMCs) a strong transcriptional response. CD8 antiviral factor activity plays a role in the control or prevention of HIV-1 infection by a non-cytolytic mechanism. The ability of Tα1 to modulate the release of antiretroviral soluble factors by CD8(+) cells was investigated. METHODS: Supernatants from lipopolysaccharide (LPS) stimulated CD8(+)-isolated cells treated with Tα1 were screened on in vitro infection of human monocyte-derived macrophages (MDMs) and PBMCs with HIV-1, and of PBMCs with human T lymphotropic virus 1 (HTLV-1). In CD8(+) cells, as well as in PBMCs of healthy donors as from HIV(+) individuals, a microarray analysis to assess the transcriptional response after treatment was performed. RESULTS: Tα1 potentiates the release, in LPS-stimulated CD8(+) cells, of soluble factors able to inhibit both in vitro HIV-1 infection of MDMs and PBMCs and in vitro HTLV-1 infection of PBMCs. A distinctive transcriptional profile was induced by Tα1 in PBMCs from HIV(+) donors. CONCLUSIONS: These findings suggest that Tα1 would represent a re-evaluated approach to antiretroviral therapy in combination with innovative treatments and with vaccine administration.
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Authors | Claudia Matteucci, Antonella Minutolo, Michela Pollicita, Emanuela Balestrieri, Sandro Grelli, Gabriella D'Ettorre, Vincenzo Vullo, Ilaria Bucci, Alessandra Luchini, Stefano Aquaro, Paola Sinibaldi-Vallebona, Beatrice Macchi, Carlo Federico Perno, Antonio Mastino, Enrico Garaci |
Journal | Expert opinion on biological therapy
(Expert Opin Biol Ther)
Vol. 15 Suppl 1
Pg. S83-100
( 2015)
ISSN: 1744-7682 [Electronic] England |
PMID | 26094546
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antiviral Agents
- CD8 Antigens
- Thymosin
- Thymalfasin
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Topics |
- Antiviral Agents
(pharmacology, therapeutic use)
- CD8 Antigens
(immunology, metabolism)
- Deltaretrovirus Infections
(drug therapy, immunology, metabolism)
- Drug Synergism
- HIV Infections
(immunology)
- HIV-1
(drug effects, immunology)
- Human T-lymphotropic virus 1
(drug effects, immunology, metabolism)
- Humans
- Leukocytes, Mononuclear
(drug effects, immunology, metabolism)
- Thymalfasin
- Thymosin
(analogs & derivatives, pharmacology, therapeutic use)
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