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Quantification of hypoglycin A in serum using aTRAQ(®) assay.

AbstractBACKGROUND:
Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication.
METHODS:
Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition.
RESULTS:
Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50μmol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals.
CONCLUSIONS:
The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
AuthorsFrançois Boemer, Michelle Deberg, Roland Schoos, Etienne Baise, Hélène Amory, Gilbert Gault, Jeremy Carlier, Yvan Gaillard, Christel Marcillaud-Pitel, Dominique Votion
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 997 Pg. 75-80 (Aug 01 2015) ISSN: 1873-376X [Electronic] Netherlands
PMID26094208 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Hypoglycins
  • hypoglycin
Topics
  • Animals
  • Horse Diseases (blood)
  • Horses
  • Hypoglycins (blood)
  • Isotope Labeling
  • Linear Models
  • Mass Spectrometry (methods)
  • Muscular Diseases (blood)
  • Reproducibility of Results
  • Sensitivity and Specificity

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