Abstract | BACKGROUND:
Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50μmol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
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Authors | François Boemer, Michelle Deberg, Roland Schoos, Etienne Baise, Hélène Amory, Gilbert Gault, Jeremy Carlier, Yvan Gaillard, Christel Marcillaud-Pitel, Dominique Votion |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 997
Pg. 75-80
(Aug 01 2015)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 26094208
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Horse Diseases
(blood)
- Horses
- Hypoglycins
(blood)
- Isotope Labeling
- Linear Models
- Mass Spectrometry
(methods)
- Muscular Diseases
(blood)
- Reproducibility of Results
- Sensitivity and Specificity
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