C-terminal neurogranin is increased in cerebrospinal fluid but unchanged in plasma in Alzheimer's disease.

Abstract

INTRODUCTION:

Biomarkers monitoring synaptic degeneration/loss would be valuable for Alzheimer's disease (AD) diagnosis. Postsynaptic protein neurogranin may be a promising cerebrospinal fluid (CSF) biomarker but has not yet been evaluated as a plasma biomarker.

METHODS:

Using an in-house designed prototype enzyme-linked immunosorbent assay (ELISA) targeting neurogranin C-terminally, we studied neurogranin in paired CSF/plasma samples of controls (n = 29) versus patients experiencing MCI, or dementia, due to AD (in total n = 59).

RESULTS:

CSF neurogranin was increased in AD and positively correlated with CSF tau, whereas there was a negative relationship between CSF neurogranin (and tau) and CSF Aβ1-42/Aβ1-40. No differences were detected in plasma neurogranin between controls and AD. Also, there was no correlation between CSF and plasma neurogranin, excluding confounding effects of the latter.

DISCUSSION:

This study strengthens the potential of neurogranin as an AD CSF biomarker, which now needs validation in larger studies. As tools, straightforward immunoassays can be used, as demonstrated by the described ELISA.

• Authors: Ann De Vos, Dirk Jacobs, Hanne Struyfs, Erik Fransen, Kerstin Andersson, Erik Portelius, Ulf Andreasson, Didier De Surgeloose, Daniëlle Hernalsteen, Kristel Sleegers, Caroline Robberecht, Christine Van Broeckhoven, Henrik Zetterberg, Kaj Blennow, Sebastiaan Engelborghs, Eugeen Vanmechelen
• Journal: Alzheimer's & dementia : the journal of the Alzheimer's Association (Alzheimers Dement) Vol. 11 Issue 12 Pg. 1461-1469 (Dec 2015) ISSN: 1552-5279 [Electronic] United States
• PMID: 26092348 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Biomarkers
• Peptide Fragments
• tau Proteins
• Neurogranin

Topics

• Adult
• Aged
• Aged, 80 and over
• Alzheimer Disease (blood, cerebrospinal fluid)
• Amyloid beta-Peptides (cerebrospinal fluid)
• Biomarkers (blood, cerebrospinal fluid)
• Cognitive Dysfunction (cerebrospinal fluid)
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Male
• Middle Aged
• Neurogranin (blood, cerebrospinal fluid)
• Peptide Fragments (cerebrospinal fluid)
• tau Proteins (cerebrospinal fluid)