Abstract |
The histologic diagnosis of Kaposi sarcoma (KS) can be confirmed with human herpes virus 8 (HHV8) latency-associated nuclear antigen (LNA)-1 immunohistochemistry, which may show variability in distribution and intensity. This retrospective study was aimed at addressing the factors that may contribute to this variability. All cases of mucocutaneous KS diagnosed in a 5-year period at the histopathology department at a tertiary hospital in South Africa with available patients' CD4 counts and HHV8 LNA-1 immunohistochemically stained slides were reviewed, and the biopsy stages of KS (patch/plaque/nodular), CD4 counts, immunohistochemistry staining method (manual vs. automated), and distribution (diffuse/focal) and intensity (strong/weak) of HHV8 LNA-1 staining were recorded. A total of 127 cases were reviewed. No relationship was demonstrated between the median CD4 count and the histologic stages of KS (P = 0.701) or the intensity and distribution of HHV8 immunohistochemical staining using either staining method. Multivariate analysis showed that method of immunohistochemical staining was a significant predictor of distribution (P = 0.006) and intensity (P = 0.044) of staining, and that stage was a significant predictor of distribution of staining (P = 0.033).
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Authors | Reena D Mohanlal, Sugeshnee Pather |
Journal | The American Journal of dermatopathology
(Am J Dermatopathol)
Vol. 37
Issue 7
Pg. 530-4
(Jul 2015)
ISSN: 1533-0311 [Electronic] United States |
PMID | 26091511
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- Nuclear Proteins
- Phosphoproteins
- latent nuclear antigen (LNA)
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Topics |
- AIDS-Related Opportunistic Infections
(complications, immunology)
- Antigens, Viral
(analysis)
- CD4 Lymphocyte Count
- Cross-Sectional Studies
- Herpesviridae Infections
(complications)
- Herpesvirus 8, Human
- Humans
- Immunohistochemistry
(methods)
- Nuclear Proteins
(analysis)
- Phosphoproteins
(analysis)
- Retrospective Studies
- Sarcoma, Kaposi
(chemistry, immunology, pathology)
- Skin Neoplasms
(chemistry, immunology, pathology)
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