[Alkaloids from Macleaya cordata and their cytotoxicity assay].

Abstract

OBJECTIVE:

To study the alkaloids of Macleaya cordata and their anti-tumor activities.

METHOD:

Alcohol and liquid-liquid extraction were used methods were used to extract the alkaloids constituents, and silica gel, reverse-phase octadecylsilyl (ODS), sephadex LH-20 chromatographic methods and HPLC were applied to isolate and purify compounds. MS, NMR spectroscopic methods were used to determine their structures. Furthermore, the cytotoxicity of these chemical components for MCF-7 and SF-268 cell lines was measured by MTT method.

RESULT:

Twelve alkaloids were isolated from the fruits of M. cordata, and their structures were identified as: maclekarpine E (1), 6-acetonyldihyrochelerythrine (2), cavidilinine (3), 6-acetonyldihyrosanguinnarine (4), O-methylzanthoxyline (5), 6-methoxy-dihydrosanguinarine (6), spallidamine (7), 6-hydroxyldihydrochelerythrine (8), arnotianamida (9), dihydrosanguinarine (10), protopine (11), and cryptopine (12).

CONCLUSION:

Compounds 1, 3, 7-9 were isolated from M. cordata for the first time, and compound 5 is a new natural product. The results of cytotoxic assay indicated that compound 6 showed strong cytotoxicity against MCF-7 and SF-268 cell lines with IC50 values of 0.61 μmol · L(-1) and 0.54 μmol · L(-1), respectively.

• Authors: Hui-liang Zou, Hong-yu Li, Shao-fu Yu, Miao Cheng, Xing-dong Zhou, Ying Zhang, Bai-lian Liu, Guang-xiong Zhou
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 40 Issue 3 Pg. 458-62 (Feb 2015) ISSN: 1001-5302 [Print] China
• PMID: 26084170 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Antineoplastic Agents, Phytogenic

Topics

• Alkaloids (isolation & purification, pharmacology)
• Antineoplastic Agents, Phytogenic (isolation & purification)
• Cell Line, Tumor
• Humans
• Papaveraceae (chemistry)