[Nongenomic effects of estrogen on extracellular signal-regulated kinases through initiating transient calcium flux in endometrial cancer].

Abstract

OBJECTIVE:

To study the mechanism on extracellular signal-regulate kinases (ERK) signal transduction by calcium influx initiated by combination of estrogen with calcium channels or estrogen receptor in endometrial cancer cell Ishikawa.

METHODS:

Confocal test was used to determine the relative calcium mobilization by stimulation of estrodiol together with and without the inhibition of ICI182780 and nifedipine. Western-blotting was used to detect the protein expression of phosphorylated ERK1/2 (P-ERK1/2) in the same condition.

RESULTS:

The transient calcium flux initiated by 17β-estrodiol (E2) and a membrane-impermeable conjugate of estrogen and bovine serum albumin (E2-BSA), and the calcium mobilization could be inhibited by ICI182780 and nifedipine in 1 min. In Ishikawa cells, phosphorylation of ERK1/2 was stimulated by E2, and the phosphorylation could not be inhibited by E2 after the combination with ICI182780 in 5 min and in 30 min. The phosphorylation also could not be inhibited by E2-BSA after the combination with nifedipine in 5 min, but in 30 min the phosphorylation was decreased. The phosphorylation of ERK by E2-BSA was decreased by the combination with nifedipine in 30 min.

CONCLUSION:

The transient calcium flux initiated by estrogen has an effect on the activation of ERK signal pathway in endometrial carcinoma cells.

• Authors: Li-li Zhang, Jian-liu Wang
• Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (Beijing Da Xue Xue Bao Yi Xue Ban) Vol. 47 Issue 3 Pg. 489-93 (Jun 18 2015) ISSN: 1671-167X [Print] China
• PMID: 26080881 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Estrogen Receptor Antagonists
• Estrogens
• Receptors, Estrogen
• estradiol-bovine serum albumin
• Fulvestrant
• Serum Albumin, Bovine
• Estradiol
• Extracellular Signal-Regulated MAP Kinases
• Nifedipine
• Calcium

Topics

• Calcium (metabolism)
• Calcium Channel Blockers (pharmacology)
• Calcium Signaling
• Endometrial Neoplasms (metabolism)
• Estradiol (analogs & derivatives, pharmacology)
• Estrogen Receptor Antagonists (pharmacology)
• Estrogens (pharmacology)
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Female
• Fulvestrant
• Humans
• Nifedipine (pharmacology)
• Phosphorylation
• Receptors, Estrogen (antagonists & inhibitors)
• Serum Albumin, Bovine (pharmacology)