SMAD4 gene promoter mutations in patients with thyroid tumors.

Abstract

As a key component of the transforming growth factor beta (TGFB) pathway, which regulates the expression of thyroid-specific genes, tumor suppressor SMAD4 is crucial for thyroid development and function. Aberrant expression of SMAD4 in thyroid tumor tissue was reported and mutations affecting the coding region have been detected, but a potential role of mutations in SMAD4 gene regulatory regions remains unexplored. The aim of this study was to analyze SMAD4 gene promoters in thyroid tumors. A total of 76 thyroidectomy specimens were studied, including 42 malignant and 34 benign tumors. The presence of mutations in four SMAD4 gene promoters was analyzed in thyroid tumor tissue and peripheral blood by PCR and DNA sequencing. The expression and intracellular localization of endogenous SMAD4 protein in selected tumor samples was studied by immunostaining and confocal microscopy. Of three novel variants detected, two were within promoter A (-204T/C and -5C/T) and one in promoter D (-180delA). Unlike somatic mutations previously detected in the nearby region, germline mutation -180delA in promoter D doesn't appear to affect SMAD4 expression in the thyroid tumor tissue. However, all newly detected SMAD4 promoter variants affect predicted binding sites of transcription factors involved in cell cycle regulation and should be further characterized functionally. Although not directly involved in carcinogenesis, detected variants may alter SMAD4 transcriptional regulation to some extent. Considering that dosage dependence is of great importance for the role of SMAD4 protein as a tumor suppressor, potential clinical significance of SMAD4 gene promoter mutations is worth further investigation.

• Authors: Aleksandra Nikolic, Momcilo Ristanovic, Vladan Zivaljevic, Aleksandra Divac Rankov, Dragica Radojkovic, Ivan Paunovic
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 99 Issue 1 Pg. 100-3 (Aug 2015) ISSN: 1096-0945 [Electronic] Netherlands
• PMID: 26079547 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• SMAD4 protein, human
• Smad4 Protein
• Transcription Factors

Topics

• Adult
• Female
• Gene Expression Regulation, Neoplastic
• Genetic Variation
• Genotyping Techniques
• Humans
• Male
• Middle Aged
• Mutation
• Promoter Regions, Genetic
• Smad4 Protein (genetics, metabolism)
• Thyroid Gland (pathology)
• Thyroid Neoplasms (genetics)
• Transcription Factors