O-2'-hydroxypropyltrimethyl ammonium chloride chitosan nanoparticles for the delivery of live Newcastle disease vaccine.

Abstract

A novel complex chitosan derivative, O-2'-hydroxypropyltrimethyl ammonium chloride chitosan (O-2'-HACC), was synthesized and used to make nanoparticles as a delivery vehicle for live attenuated Newcastle disease vaccine. We found that O-2'-HACC had high antimicrobial activity, low toxicity, and a high safety level. Newcastle disease virus (NDV) was then encapsulated in the O-2'-HACC nanoparticles (NDV/La Sota-O-2'-HACC-NPs) by the ionic crosslinking method, and the properties of the resulting nanoparticles were determined by transmission electron microscopy, Zeta potential analysis, Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray diffraction. NDV/La Sota-O-2'-HACC-NPs had regular spherical morphologies and high stability, with an encapsulation efficiency of 95.68 ± 2.2% and a loading capacity of 58.75 ± 4.03%. An in vitro release assay indicated that release of NDV from NDV/La Sota-O-2'-HACC-NPs occurred slowly. Specific pathogen-free chickens immunized with NDV/La Sota-O-2'-HACC-NPs intranasally had much stronger cellular, humoral and mucosal immune responses than did those immunized intramuscularly or with live attenuated Newcastle disease vaccine. NDV/La Sota-O-2'-HACC-NPs are a novel drug delivery carrier with immense potential in medical applications.

• Authors: Chunxiao Dai, Hong Kang, Wanqiu Yang, Jinyan Sun, Chunlong Liu, Guogang Cheng, Guangyu Rong, Xiaohua Wang, Xin Wang, Zheng Jin, Kai Zhao
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 130 Pg. 280-9 (Oct 05 2015) ISSN: 1879-1344 [Electronic] England
• PMID: 26076628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• Drug Carriers
• Quaternary Ammonium Compounds
• Viral Vaccines
• hydroxypropyltrimethyl ammonium chloride chitosan
• Chitosan

Topics

• Animals
• Cells, Cultured
• Chickens (immunology)
• Chitosan (analogs & derivatives, chemistry, pharmacology)
• Cytokines (metabolism)
• Drug Carriers
• Drug Delivery Systems
• Microscopy, Electron, Transmission
• Nanoparticles (chemistry)
• Newcastle Disease (immunology, prevention & control)
• Newcastle disease virus (immunology)
• Poultry Diseases (immunology, prevention & control)
• Proton Magnetic Resonance Spectroscopy
• Quaternary Ammonium Compounds (chemistry, pharmacology)
• Spectroscopy, Fourier Transform Infrared
• Vaccination (methods, veterinary)
• Viral Vaccines (therapeutic use)
• X-Ray Diffraction