Distribution and peroxidative oxidation of 2-t-butyl-4-methoxyphenol in rat tissues after a single intraperitoneal dose.

Abstract

The distribution of 2-t-butyl-4-methoxyphenol (BHA) and its conversion to 2,2'-dihydroxy-3,3'-di-t-butyl-5,5'-dimethoxydiphenyl (di-BHA) in rat tissues at different times (1-96 hr) following the intraperitoneal administration of a single dose of BHA (32 mg kg-1 body weight) were monitored by gas chromatography-mass spectrometry (GC/MS) analysis of both compounds. High BHA levels were found in the intestine and liver persisting up to 24 hours (5.5-20.7 and 1.8-3.3 micrograms g-1 wet weight, respectively). In these tissues, values of the area under the experimental concentration curve (AUC0-24) were 285 and 49 times higher, respectively, than those observed in plasma (945 ng mL-1 hr), AUC0-24 values in kidney, spleen, erythrocytes, and brain were 2-7 times higher, whereas values below those found in plasma were observed in lung and muscle. The metabolite di-BHA could be detected in the intestine, kidney, and spleen, amounting to 5-8% of BHA. These findings indicate that rat intestine is capable of transforming in vivo BHA into di-BHA even when the former compound is administered intraperitoneally and that this capacity is shared by the kidney and spleen.

• Authors: L Della Corte, L Bianchi, M Valoti, G Sgaragli
• Journal: Journal of biochemical toxicology (J Biochem Toxicol) Vol. 4 Issue 3 Pg. 147-50 ( 1989) ISSN: 0887-2082 [Print] United States
• PMID: 2607540 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peroxides
• Butylated Hydroxyanisole

Topics

• Animals
• Biotransformation
• Butylated Hydroxyanisole (administration & dosage, metabolism, pharmacokinetics)
• Gas Chromatography-Mass Spectrometry
• Injections, Intraperitoneal
• Male
• Oxidation-Reduction
• Peroxides (metabolism)
• Rats
• Rats, Inbred Strains
• Tissue Distribution