Analytical and physicochemical characterisation of the senile cataract drug dipeptide β-alanyl-L-histidine (carnosine).
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| Abstract |
This study presents a simple but sensitive HPLC chromatographic method with a stability-indicating assay for determination and physicochemical characterisation of L-carnosine, a promising senile cataract prophylactic agent. Chromatographic analysis was conducted using a reverse phase (RP)-HPLC system and an isocratic mobile phase of 98% v/v trifluoroacetic acid (0.1% v/v) and 2% v/v acetonitrile with detection at 220 nm. L-carnosine was subjected to stress conditions to force its degradation using chemical and thermal agents and was subsequently detected from its degradation products using ESI-MS. The lipophilicity of the drug and 1:1 drug to phospholipid complex (PC) mol/mol was determined by estimating the partition coefficient (P). Lipophilicity was greatly enhanced when L-carnosine was formulated as a phospholipid complex using the solvent evaporation method. L-carnosine-phospholipid complex could be a promising approach for effective delivery to the human lens as offers a potential novel treatment for senile cataract.
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| Authors | Hamdy Abdelkader, Julian Swinden, Barbara K Pierscionek, Raid G Alany |
| Journal | Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 114 Pg. 241-6 (Oct 10 2015) ISSN: 1873-264X [Electronic] England |
| PMID | 26073114 (Publication Type: Journal Article) |
| Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
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