Oncoleaking: Use of the Pore-Forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy.

Abstract

Suicide gene therapy has been shown to be very efficient in tumor eradication. Numerous suicide genes were tested in vitro and in vivo demonstrating their therapeutic potential in clinical trials. Apart from this, still growing efforts are made to generate more targeted and more effective suicide gene systems for cancer gene therapy. In this regard bacterial toxins are an alternative, which add to the broad spectrum of different suicide strategies. In this context, the claudin-targeted bacterial Clostridium perfringens enterotoxin (CPE) is an attractive new type of suicide oncoleaking gene, which as pore-forming protein exerts specific and rapid toxicity towards claudin-3- and -4-overexpressing cancers. In this chapter we describe the generation and use of CPE-expressing vectors for the effective tumor cell killing as novel suicide gene approach particularly for treatment of therapy refractory tumors.

• Authors: Jessica Pahle, Jutta Aumann, Dennis Kobelt, Wolfgang Walther
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1317 Pg. 69-85 ( 2015) ISSN: 1940-6029 [Electronic] United States
• PMID: 26072402 (Publication Type: Journal Article)

Chemical References

• Claudins
• Enterotoxins
• enterotoxin, Clostridium
• L-Lactate Dehydrogenase

Topics

• Blotting, Western
• Bystander Effect
• Cell Line, Tumor
• Claudins (chemistry, metabolism)
• Clostridium perfringens (metabolism)
• Enterotoxins (genetics, therapeutic use)
• Enzyme-Linked Immunosorbent Assay
• Genes, Transgenic, Suicide
• Genetic Therapy (methods)
• Genetic Vectors (metabolism)
• Humans
• Immunohistochemistry
• L-Lactate Dehydrogenase (metabolism)
• Real-Time Polymerase Chain Reaction
• Transfection