Estimation of TiO₂ nanoparticle-induced genotoxicity persistence and possible chronic gastritis-induction in mice.

Abstract

Titanium dioxide (TiO2) nanoparticles are widely used as a food additive and coloring agent in many consumer products however limited data is available on the nano-TiO2 induced genotoxicity persistence. Thus, this study investigated the persistence of nano-TiO2 induced genotoxicity and possible induction of chronic gastritis in mice. The mice were orally administered 5, 50 or 500 mg/kg body weight nano-TiO2 for five consecutive days, and then mice from each dosage group were sacrificed 24 h or one or two weeks after the last treatment. The administration of nano-TiO2 resulted in persistent apoptotic DNA fragmentation and mutations in p53 exons (5-8) as well as significant persistent elevations in malondialdehyde and nitric oxide levels and decreases in the reduced glutathione level and catalase activity compared with the control mice in a dose- and time-dependent manner. Necrosis and inflammation were evident upon histological examination. These findings could be attributed to the persistent accumulation of nano-TiO2 at the tested doses at all three time points. Based on these findings, we conclude that the administration of nano-TiO2, even at low doses, leads to persistent accumulation of nano-TiO2 in mice, resulting in persistent inflammation, apoptosis and oxidative stress, ultimately leading to the induction of chronic gastritis.

• Authors: Hanan Ramadan Hamad Mohamed
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 83 Pg. 76-83 (Sep 2015) ISSN: 1873-6351 [Electronic] England
• PMID: 26072100 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Biomarkers
• Gastrointestinal Agents
• Mutagens
• Water Pollutants, Chemical
• titanium dioxide
• Titanium

Topics

• Administration, Oral
• Animals
• Apoptosis (drug effects)
• Biomarkers (metabolism)
• DNA Fragmentation
• Dose-Response Relationship, Drug
• Gastric Mucosa (drug effects, immunology, metabolism, pathology)
• Gastritis (chemically induced, immunology, metabolism, pathology)
• Gastrointestinal Agents (administration & dosage, toxicity)
• Male
• Metal Nanoparticles (administration & dosage, toxicity)
• Mice, Inbred Strains
• Mutagens (administration & dosage, toxicity)
• Necrosis
• Oxidative Stress (drug effects)
• Random Allocation
• Tissue Distribution
• Titanium (administration & dosage, toxicity)
• Toxicokinetics
• Water Pollutants, Chemical (administration & dosage, toxicity)